"AGLU" Related Products

alpha-Glucosidase Assay Kit

Cat.No.: Kit-0062
Product Overview: alpha-Glucosidase Assay Kit is a colorimetric kinetic determination of a-Glucosidase activity.
Description: α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-Dglucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. alpha-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-Dglucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Applications: Direct Assays: α-glucosidase activity in biological samples.Characterization and Quality Control for α-glucosidase production.Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.
Usage: For research use only (RUO)
Storage: Store all reagents at -20°C. Shelf life: 6 months after receipt.
Kit Components: Assay Buffer: (pH 7.0) 24 mLα-NPG Substrate: 1 mLCalibrator: (equivalent to 250 U/L) 10 mL
Detection method: Colorimetric
Compatible Sample Types: Biological Sample
Features & Benefits: High sensitivity and wide linear range: Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L.Homogeneous and simple procedure: Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.Robust and amenable to HTS: All reagents are compatible with highthroughput liquid handling instruments.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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