Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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alpha-Glucosidase Assay Kit

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Cat.No.:
Kit-0062
Product Overview:
alpha-Glucosidase Assay Kit is a colorimetric kinetic determination of a-Glucosidase activity.
Description:
α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-Dglucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. alpha-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-Dglucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Applications:
Direct Assays: α-glucosidase activity in biological samples.Characterization and Quality Control for α-glucosidase production.Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.
Usage:
For research use only (RUO)
Storage:
Store all reagents at -20°C. Shelf life: 6 months after receipt.
Kit Components:
Assay Buffer: (pH 7.0) 24 mLα-NPG Substrate: 1 mLCalibrator: (equivalent to 250 U/L) 10 mL
Detection method:
Colorimetric
Compatible Sample Types:
Biological Sample
Features & Benefits:
High sensitivity and wide linear range: Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L.Homogeneous and simple procedure: Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.Robust and amenable to HTS: All reagents are compatible with highthroughput liquid handling instruments.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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