alpha-Glucosidase Assay Kit is a colorimetric kinetic determination of a-Glucosidase activity.
α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-Dglucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. alpha-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-Dglucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Direct Assays: α-glucosidase activity in biological samples.Characterization and Quality Control for α-glucosidase production.Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.
For research use only (RUO)
Store all reagents at -20°C. Shelf life: 6 months after receipt.
Assay Buffer: (pH 7.0) 24 mLα-NPG Substrate: 1 mLCalibrator: (equivalent to 250 U/L) 10 mL
Compatible Sample Types:
Features & Benefits:
High sensitivity and wide linear range: Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L.Homogeneous and simple procedure: Simple "mix-and-measure" procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.Robust and amenable to HTS: All reagents are compatible with highthroughput liquid handling instruments.