Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
"cAMP" Related Products.
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Product Overview:
The kit can detect ~0.1-10 pmol/5 µl (or ~ 0.02-2 µM) cAMP samples. (100 assays) Simple procedureFast and convenientThe assay is sensitive, stable and high-throughput adaptableThe kit provides a new acetylation procedure to improve detection sensitivity significantly
100 assays
Adenosine 3",5"-cyclic monophosphate (cyclic AMP, cAMP) is an important "second messenger" involved in many physiological processes. cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody onto the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the cAMP concentration in samples. The kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/well (or ~ 0.02 -2 µM) cAMP samples.
The kit can detect ~0.1-10 pmol/5 µl (or ~ 0.02-2 µM) cAMP samples.
Target Species:
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components:
10X cAMP Assay BufferStandard cAMP (10 nmol)Neutralizing BufferAcetylating Reagent AAcetylating Reagent BRabbit Anti-cAMP pAbHRP DeveloperProtein G Coated Plate
Detection method:
Compatible Sample Types:
Cell Culture SupernatantsPlasmaSerumTissue LysatesUrineCell Lysates

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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