Active Recombinant Human IL2 Protein, His-tagged

• Cat.No.: IL2-636H
• Product Overview: Human IL2 (133 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).

Specification

• Description: This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Source: Plants
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Bioactivity of recombinant human IL2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically < 0.25 ng/mL, corresponding to a specific activity > 4×10 6 U/mg.
• Endotoxin: < 0.005 ng/μg protein (0.05 EU/μg) as measured by turbidimetric kinetic assay
• Purity: > 98% by SDS-PAGE
• Applications: Western Blot (Recombinant protein)
  Functional Study
  SDS-PAGE
• Storage: Store at -20 centigrade on dry atmosphere.
  After reconstitution with sterile water to a concentration of no less than 100 μg/mL, store at -20 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Aliquot to avoid repeated freezing and thawing.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from PBS, pH 7.2
• Reconstitution: Sterile water

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens (human) ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; IL-2; TCGF; lymphokine; interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.