| Cat. No. : |
AF251705-534M |
| Description : |
AF251705, also known as CMRF35A, CD300c, CLM4, DIgR1, and MAIRII,is a 35 kDa type I transmembrane glycoprotein that belongs to theimmunoregulatory signaling (IRS) family of immunoglobulinlike molecules.Mature mouse AF251705 consists of a 165amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aatransmembrane segment, and a 20 aa cytoplasmic tail. An alternate splice formlacks two amino acids C-terminal to the Ig-like domain. Within the ECD, mouseAF251705 shares 45% aa sequence identitywith human AF251705. |
| Source : |
Chinese HamsterOvary cell line, CHO-derived. |
| Form : |
Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence : |
Cys22 |
| Molecular Weight : |
46 kDa(monomer) |
| Activity : |
Measuredby its ability to inhibit anti-CD3-induced proliferation of stimulated human Tcells. Human T lymphocytes cultured for 72 hours with PHA were incubated foran additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 andserially diluted rmLMIR-2/Fc. The ED50 for this effect istypically 0.6-3 μg/mL. |
| Endotoxin Level : |
< 1.0EU per 1 μg of the protein by the LAL method. |
| SDS-PAGE : |
55-65 kDa,reducing conditions. |
| Purity : |
> 95%,by SDSPAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
100μg/mL in PBS. |
| Storage : |
Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
