Mouse Anti-Human EGFR Monoclonal Antibody

• Cat.No.: CAB-11472MH
• Product Overview: Anti-human EGFR mAb,is derived from hybridization of mouse F0 myeloma cells with spleen cellsfrom BALB/c mice immunized with a recombinant human EGFR protein.

Specification

• Cat. No.: CAB-11472MH
• Antigen Description: Epidermal growthfactor receptor (EGFR) is a 170 kDa membrane protein first identified as abinding partner of EGF. Also, EGFR phosphorylated itself(autophosphorylation) in response to EGF treatment. The overexpression of EGFRin various epithelial tumors was widely reported and it has beensubstantiated that deregulation of EGFR itself and its signaling pathways animportant role in human cancers.
• Immunogen: Recombinant humanEGFR (424-605aa) purified from E. coli.
• Host: Mouse
• Isotype: MouseIgG2b heavy chain and κ light chain.
• Application: ELISA; WB;ICC
• Clone: BU3I9
• Description: EGFR isthe cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.
• Concentration: 1 mg/ml
• Form: Liquid.In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
• Usage: Theantibody has been tested by ELISA, Western blot analysis to assurespecificity and reactivity. Since application varies, however, eachinvestigation should be titrated by the reagent to obtain optimal results.Recommended dilution range for Western blot analysis is 1:250.Immunofluorescence is 1:250. Recommended starting dilution is 1:250.
• Storage: Can bestored at +4°C. For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: EGFR epidermalgrowth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR;epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61;proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cellproliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1;avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; EC 2.7.10.1;OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663;OTTHUMP00000209210; OTTHUMP00000209211; EC 2.7.10; epidermal growth factorreceptor (avian erythroblastic leukemia viral (v-erb-b); erythroblasticleukemia viral (v-erb-b) oncogene homolog; leukemia viral (v-erb-b) oncogenehomolog
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: Adherens junction; Alpha6-Beta4Integrin Signaling Pat; Androgen Receptor Signaling Pathway; Arf6 signalingevents; Axon guidance; Bladder cancer; Calcium signaling pathway;Cytokine-cytokine receptor interaction; Delta-Notch Signaling Pathway;Developmental Biology; Direct p53 effectors; Dorso-ventral axis formation;E-cadherin signaling in keratinocytes; EGF receptor (ErbB1) signalingpathway; EGFR downregulation
• Function: ATP binding; MAP/ERK kinase kinaseactivity; actin filament binding; double-stranded DNA binding; enzymebinding; epidermal growth factor-activated receptor activity; identicalprotein binding; contributes to nitric-oxide synthase regulator activity;nucleotide binding; protein binding; protein heterodimerization activity;protein phosphatase binding; protein tyrosine kinase activity; receptoractivity; signal transducer activity

Reviews

09/05/2022

The powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.

09/26/2019

Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.

03/23/2019

The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.

Q&As

What biological processes of human embryonic development and tissue regeneration is EGFR associated with?

It is associated with biological processes in human embryonic development and tissue regeneration.

Why does EGFR-inhibiting drug therapy have adverse reactions?

EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.

What is the role of EGFR in tumor treatment?

The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.

What are the general methods used for EGFR gene mutation testing?

EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.

What other drugs can EGFR inhibitors be combined with to increase efficacy?

The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.

How do EGFR mutations affect the efficacy of targeted therapies?

EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.