Anti-human EGFR mAb,is derived from hybridization of mouse F0 myeloma cells with spleen cellsfrom BALB/c mice immunized with a recombinant human EGFR protein.
Epidermal growthfactor receptor (EGFR) is a 170 kDa membrane protein first identified as abinding partner of EGF. Also, EGFR phosphorylated itself(autophosphorylation) in response to EGF treatment. The overexpression of EGFRin various epithelial tumors was widely reported and it has beensubstantiated that deregulation of EGFR itself and its signaling pathways animportant role in human cancers.
Recombinant humanEGFR (424-605aa) purified from E. coli.
MouseIgG2b heavy chain and κ light chain.
EGFR isthe cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.
Liquid.In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
Theantibody has been tested by ELISA, Western blot analysis to assurespecificity and reactivity. Since application varies, however, eachinvestigation should be titrated by the reagent to obtain optimal results.Recommended dilution range for Western blot analysis is 1:250.Immunofluorescence is 1:250. Recommended starting dilution is 1:250.
Can bestored at +4°C. For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.