Rabbit Monoclonal Antibody to Human Epidermal Growth Factor Receptor
As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, the subsequent tyrosine autophosphorylation and initiates various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression , and thus applications focused on EGFR was proposed as a target for cancer therapy currently.
Human EGFR / ErbB / HER1. No cross-reactivityin ELISA with Human ErbB2 / HER2; Human ErbB3 / HER3; Human cell lysate (293 cell line)
Recombinant Human EGFR Protein
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB. Using a DAB detection system, the detection limit for Human EGFR is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.00245 ng/well.
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human EGFR
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.