Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Rabbit Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody

Rabbit Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody

   Share
"EGFR" Related Products.
Cat.No.:
CAB11528RH
Product Overview:
Rabbit Monoclonal Antibody to Human Epidermal Growth Factor Receptor
Antigen Description:
As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, the subsequent tyrosine autophosphorylation and initiates various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression , and thus applications focused on EGFR was proposed as a target for cancer therapy currently.
Specificity:
Human EGFR / ErbB / HER1. No cross-reactivityin ELISA with Human ErbB2 / HER2; Human ErbB3 / HER3; Human cell lysate (293 cell line)
Immunogen:
Recombinant Human EGFR Protein
Host:
Rabbit
Isotype:
Rabbit IgG
Species:
Human
Clone:
A022
Applications:
WB; ELISA
Dilution:
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB. Using a DAB detection system, the detection limit for Human EGFR is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.00245 ng/well.
Preparation:
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human EGFR
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.