Rabbit Anti-Human Epidermal Growth Factor Receptor Monoclonal Antibody

• Cat.No.: CAB11528RH
• Product Overview: Rabbit Monoclonal Antibody to Human Epidermal Growth Factor Receptor

Specification

• Antigen Description: As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, the subsequent tyrosine autophosphorylation and initiates various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression , and thus applications focused on EGFR was proposed as a target for cancer therapy currently.
• Specificity: Human EGFR / ErbB / HER1. No cross-reactivityin ELISA with Human ErbB2 / HER2; Human ErbB3 / HER3; Human cell lysate (293 cell line)
• Immunogen: Recombinant Human EGFR Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A022
• Applications: WB; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB. Using a DAB detection system, the detection limit for Human EGFR is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.00245 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human EGFR
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533
• Chromosome Location: 7p12
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem
• Function: ATP binding; MAPK/ERK kinase kinase activity; actin filament binding; double-stranded DNA binding; enzyme binding; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; identical protein binding; contributes_to nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; signal transducer activity; transmembrane receptor protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity