Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Mouse Anti-Human Interleukin-33 Monoclonal Antibody

Mouse Anti-Human Interleukin-33 Monoclonal Antibody

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Cat.No.:
CAB11566MH
Product Overview:
Mouse Monoclonal Antibody to Human Interleukin-33
Antigen Description:
Interleukin-33(IL-33), also known as DVS 27 and NF-HEV, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyerss patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. IL-33 mediates its biological effects via Toll-interleukin 1 receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines. IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.
Specificity:
Human IL33. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen:
E.coli-derived rhIL-33
Host:
Mouse
Isotype:
MouseIgG2a
Species:
Human
Cross Reactivity:
5B4H5H9
Clone:
6C5I6I10
Applications:
WB
Dilution:
Western blot:This antibody can be used at 1 - 2μg /mL with the appropriate secondary reagents to detect IL-33 in WB. Using a DAB detection system, the detection limit for IL-33 is approximately 20ng/lane under reducing conditions and 100ng/lane non-reducing conditions.
Preparation:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human IL-33 (rhIL-33; aa 112-270; NP_002296.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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