Mouse Monoclonal Antibody to Human Interleukin-33
Interleukin-33(IL-33), also known as DVS 27 and NF-HEV, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyerss patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. IL-33 mediates its biological effects via Toll-interleukin 1 receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines. IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.
Human IL33. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Western blot:This antibody can be used at 1 - 2μg /mL with the appropriate secondary reagents to detect IL-33 in WB. Using a DAB detection system, the detection limit for IL-33 is approximately 20ng/lane under reducing conditions and 100ng/lane non-reducing conditions.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human IL-33 (rhIL-33; aa 112-270; NP_002296.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose
This antibody can be stored at 2-8 °C for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.