Catalog # or name ...
Recombinant Human CD73(Gln73Met232),fused an Hemagglutinin tag at Nterminal, was expressed in CHO Cells.
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Chinese Hamster Ovary cell.
30-35kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 104 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.63μg/mL.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDSPAGEunder reducing conditions and visualized by silver stain.
Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Use a manual defrost freezer and avoid repeated freezethawcycles.12 months from date of receipt, 20to 70oC as supplied.1 month, 2 to 8 oC under sterile conditions after reconstitution.3 months, 20to 70oC under sterile conditions after reconstitution.
CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG; HLADG; Ia-GAMMA; CD74; HLA class II histocompatibility antigen gamma chain; ii; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; ia antigen-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 antigen
Antigen processing and presentation
MHC class II protein binding; beta-amyloid binding; cytokine binding; cytokine receptor activity; identical protein binding
PDB rendering based on 1icf.
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