Recombinant Human CD74, Hemagglutinin-tagged

Cat. No.

CD74-2231H

Product Overview

Recombinant Human CD73(Gln73­Met232),fused an Hemagglutinin tag at N­terminal, was expressed in CHO Cells.

Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Source

Chinese Hamster Ovary cell.

N-terminalSequenceAnalysis

Tyr

PredictedMolecularMass

19.3 kDa

SDS-PAGE

30-35kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 104 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner.The ED50 for this effect is typically 0.63μg/mL.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Purity

>95%, by SDSPAGEunder reducing conditions and visualized by silver stain.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability &Storage

Use a manual defrost freezer and avoid repeated freezethawcycles.12 months from date of receipt, 20to 70^oC as supplied.1 month, 2 to 8 ^oC under sterile conditions after reconstitution.3 months, 20to 70^oC under sterile conditions after reconstitution.

Gene Information