Rat Anti-IL6 Monoclonal Antibody

Cat.No.: DMABT-H19253
Product Overview: Rat Anti-IL6 Monoclonal Antibody
Antigen Description: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. TheNR3-40D4antibody can neutralize the bioactivity of natural or recombinant IL-6.
Target: IL6
Immunogen: COS-7- expressed, recombinant human IL-6
Host: Rat
Isotype: IgG2a, kappa
species: Human
Clone: NR3-40D4
Conjugation: Biotin
Applications: ELISA,ELISPOT
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Usage recommendation: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to9pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C.Do not freeze.
Size: 50 μg;500 μg
Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
Official Symbol: IL6
Synonyms: IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6;
Gene ID: 3569
mRNA Refseq: NM_000600
Protein Refseq: NP_000591
MIM: 147620
UniProt ID: P05231
Chromosome Location: 7p21-p15
Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem;
Function: cytokine activity; cytokine activity; growth factor activity; interleukin-6 receptor binding; contributes_to interleukin-6 receptor binding; interleukin-6 receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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