Rat Anti-IL12B Monoclonal Antibody
Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12.
The LEAF (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT capture antibody, a concentration range of 2-6 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
The antibody solution should be stored undiluted at 4 °C. This LEAF solution contains no preservative; handle under aseptic conditions.