Active Recombinant Human HMGB1 Protein, His-tagged

• Cat.No.: HMGB1-526H
• Product Overview: Recombinant Human HMGB1 Protein (P09429) (Lys57-Asp158) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized Powder
• Bio-activity: Immobilized human His-HMGB1 at 10 μg/ml (100 μl/well) can bind AGER-Fc with a linear range of 0.31-2.5 μg/ml.
• Molecular Mass: The recombinant human HMGB1 consists of 117 amino acids and predicts a molecular mass of 13.6 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade.
• Storage Buffer: Lyophilized from sterile PBS, 20% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens ]
• Official Symbol: HMGB1
• Synonyms: HMG1; HMG3; HMG-1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429

Reviews

09/22/2021

Broad storage conditions of HMGB1ins make the experimental process more stable and reliable, which provides a strong guarantee for scientific research.

05/02/2020

I found that tHMGB1 exhibited very good stability during storage, which allowed us to perform experiments easily and obtain reliable results.

01/11/2019

The anti-contamination ability of HMGB1 was excellent in Western blot, and the contamination problem in the experiment was avoided.

Q&As

Why is HMGB1 protein associated with tumors?

This protein can promote tumor growth, invasion and angiogenesis, and plays an important role in tumor immune evasion.

Is HMGB1 protein involved in tumor metastasis?

Yes, HMGB1 protein plays an important role in tumor metastasis, particularly by promoting invasion of tumor cells and migration of the hematopoietic system.

What is the association between HMGB1 protein and cardiovascular disease?

Is involved in inflammatory response, vascular damage, and thrombosis in cardiovascular disease and may be associated with myocardial ischemia-reperfusion injury.

What is the effect of HMGB1 protein on the immune system?

HMGB1 protein can promote the activation, chemotaxis and inflammatory response of immune cells, and is also involved in regulating the balance of autoimmune response.

What role does HMGB1 play in diabetes?

HMGB1 protein is associated with processes such as chronic inflammation and insulin resistance in diabetes and may be a potential target for treatment and prevention.

Does HMGB1 play a role in liver disease?

The protein is involved in pathological processes such as liver fibrosis, hepatitis, and liver cancer in liver disease and may be a target for therapeutic strategies.