Active Recombinant Human CSF2

• Cat.No.: CSF2-23H
• Product Overview: Recombinant Human CSF2 was produced in rice suspension cells (Oryza sativa L. cv. Dongjin).

Specification

• Description: Granulocyte macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor produced by various cell types such as T and B cells, macrophages, endothelial cells and fibroblasts, in response to cytokine or inflammatory stimuli. GM-CSF stimulates the development of granulocytes and macrophages, and the proliferation and development of erythroid, megakaryocyte and eosinophil progenitors. It also enhances functional activity of mature hematopoietic cells. GM-CSF is of great clinical value in reconstitution of the hematopoietic system following cancer therapy or bone marrow transplantation.
• Source: Rice
• Species: Human
• Form: Sterile filtered through a 0,2 μm filter. Lyophilized from PBS, pH 7.2
• Bio-activity: Human GM-CSF is measured by its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect is typically below 0.2 ng/ml corresponding to specific activity of >5 x 10e6 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
• Molecular Mass: Recombinant human GM-CSF contains 127 amino acids in protein and no amino acid in tag. MW is 14KDa without glycosylation and around 26KDa with gycosylation.
• Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg) as measured by LAL assay.
• Purity: Greater than 95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; GM CSF; GMCSF; granulocyte macrophage colony stimulating factor; molgramostin; sargramostim; CSF; colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897
• Gene ID: 1437
• mRNA Refseq: NM_000758
• Protein Refseq: NP_000749
• MIM: 138960
• UniProt ID: P04141
• Chromosome Location: 5q23-q31
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding