Recombinant Rat IL3 cell lysate

• Cat.No.: IL3-445RCL
• Product Overview: Rat IL3 / interleukin 3 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Rat
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: Il3 interleukin 3 [ Rattus norvegicus ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3; interleukin-3; IL-3; MCGF; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor
• Gene ID: 24495
• mRNA Refseq: NM_031513
• Protein Refseq: NP_113701
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem
• Function: cytokine activity; cytokine activity; cytokine activity; cytokine activity; growth factor activity; growth factor activity; interleukin-3 receptor binding

Reviews

06/20/2022

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03/08/2021

The performance is very stable and the price is very reasonable. I would definitely buy this product again.

12/19/2020

I have recommended this brand to my colleagues and friends, and they all praise this product.

Q&As

What is the difference between IL-3 and GM-CSF In vitro?

In vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.

What is the difference in clinical presentation of IL-3 compared to GM-CSF?

Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.

Since the structure of IL-3 is different between animals and humans, does it mean that il-3 is not necessary to maintain stable hematopoietic function?

mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells

How does the structure of IL-3 in animals differ from that in humans?

Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.

Does loss of cytokine IL-3 affect normal granulocyte, monocyte, and basophil levels?

Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.

IL-3 specific receptor subunit CD123 is overexpressed in many hematological malignancies,Is it overexpressed in normal tissues where inflammation occurs?

non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.

When is IL-3 activated by T cells?

Such as allergic inflammation and parasitic infestation.

Since basophils are the target and source of IL-3, can they regulate the secretion of IL-3 to treat allergic diseases?

IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.

T cell IL-3 expression is specifically regulated by which transcription factor?

Bhlhe40

Which hematopoietic and non-hematopoietic cell subsets express IL-3?

E.g., lymphocytes,epithelial cells, and fibroblasts

Are there any completed clinical trials on IL3?

Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.

What is the effect of IL3 on hematopoietic system?

This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.

Are there any side effects of IL3?

In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.

IL3 associated with whether the immune adjustment?

IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.

What is the solubility and stability of IL3?

IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.

How is the research progress of IL3?

The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.