"LILRB1" Related Products

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Recombinant Human LILRB1 cell lysate

Cat.No.: LILRB1-774HCL
Product Overview: Human LILRB1 / CD85 / ILT2 / ILR1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source: Human cells
Species: Human
Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: LILRB1 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 [ Homo sapiens ]
Official Symbol: LILRB1
Synonyms: LILRB1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; leukocyte immunoglobulin-like receptor subfamily B member 1; CD85; CD85j; ILT2; LIR 1; LIR1; MIR 7; ILT-2; CD85 antigen; Ig-like transcript 2; leukocyte Ig-like receptor-1; immunoglobulin-like transcript 2; CD85 antigen-like family member J; leukocyte immunoglobulin-like receptor 1; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; MIR7; CD85J; LIR-1; MIR-7; FLJ37515;
Gene ID: 10859
mRNA Refseq: NM_001081637
Protein Refseq: NP_001075106
MIM: 604811
UniProt ID: Q8NHL6
Chromosome Location: 19q13.4
Pathway: Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem;
Function: HLA-A specific inhibitory MHC class I receptor activity; HLA-B specific inhibitory MHC class I receptor activity; MHC class I protein binding; MHC class I protein binding; MHC class I protein binding; MHC class I receptor activity; SH2 domain binding; protein homodimerization activity; protein phosphatase 1 binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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