Recombinant Human NGFR cell lysate
Cat.No. : | NGFR-1670HCL |
Product Overview : | Human NGFR/ P75 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | NGFR nerve growth factor receptor [ Homo sapiens ] |
Official Symbol : | NGFR |
Synonyms : | NGFR; nerve growth factor receptor; nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16; CD271; low affinity nerve growth factor receptor; p75NTR; TNFR superfamily; member 16; TNFRSF16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; p75(NTR); Gp80-LNGFR; |
Gene ID : | 4804 |
mRNA Refseq : | NM_002507 |
Protein Refseq : | NP_002498 |
MIM : | 162010 |
UniProt ID : | P08138 |
Chromosome Location : | 17q21-q22 |
Pathway : | Axonal growth inhibition (RHOA activation), organism-specific biosystem; Axonal growth stimulation, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Ceramide signalling, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; NADE modulates death signalling, organism-specific biosystem; |
Function : | death receptor activity; nerve growth factor binding; protein binding; receptor activity; signal transducer activity; transmembrane signaling receptor activity; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionNGFR protein is involved in the survival and maintenance of nerve cells, and its dysregulation has been associated with neurodegenerative diseases such as Alzheimer's and Parkinson's disease.
Research into NGFR protein as a potential target for the development of novel pain therapies is ongoing, with the aim of modulating pain signaling pathways.
Some studies have explored the potential of NGFR protein as a therapeutic target for neurodegenerative diseases, with the aim of promoting nerve cell survival and function.
Research suggests that alterations in NGFR protein levels may serve as a potential biomarker for neurodegenerative diseases, aiding in early diagnosis and monitoring disease progression.
NGFR protein is being investigated for its potential role in promoting nerve regeneration after injury, with the aim of developing new treatments for nerve damage and paralysis.
Customer Reviews (3)
Write a reviewIts usage in ELISA and protein electron microscopy structure analysis has been reported to yield highly reproducible and interpretable results, providing valuable insights into the molecular mechanisms and biological processes under investigation.
Its excellent performance in ELISA assays makes it a valuable tool in quantifying analytes in biological samples.
Its reliability, specificity, and versatility make it an invaluable asset in studying and understanding complex biological systems.
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