Recombinant Mouse MERTK cell lysate

• Cat.No.: MERTK-1816MCL
• Product Overview: Mouse MERTK / MER derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: Mertk c-mer proto-oncogene tyrosine kinase [ Mus musculus ]
• Official Symbol: MERTK
• Synonyms: MERTK; c-mer proto-oncogene tyrosine kinase; tyrosine-protein kinase Mer; Tyro 12; proto-oncogene c-Mer; receptor tyrosine kinase MerTK; proto-oncogene tyrosine-protein kinase Mer; Eyk; Mer; Nyk; nmf12
• Gene ID: 17289
• mRNA Refseq: NM_008587
• Protein Refseq: NP_032613
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; transmembrane receptor protein tyrosine kinase activity

Reviews

04/08/2022

Fast shipping and excellent service. This product is a lab essential.

06/15/2020

Highly recommend for quality and prompt delivery.

03/25/2018

Exceptional product, outstanding customer service!

Q&As

In cancer biology, how does MERTK contribute to tumor progression and metastasis, and are there potential therapeutic strategies targeting MERTK in cancer treatment?

MERTK's involvement in cancer includes promoting tumor cell survival and metastasis. Targeting MERTK is under investigation as a potential therapeutic strategy in cancer treatment.

How does the Gas6-MERTK signaling axis contribute to cellular processes beyond phagocytosis, and what downstream effectors are activated upon MERTK activation?

The Gas6-MERTK signaling axis influences diverse cellular processes, activating downstream effectors such as PI3K/Akt and MAPK pathways. Understanding these signaling events provides insights into MERTK's functional diversity.

In neurobiology, what is the role of MERTK in microglial phagocytosis and synaptic pruning, and how does this impact neural circuitry development?

MERTK is involved in microglial phagocytosis and synaptic pruning, contributing to neural circuitry development. Dysregulation may affect synaptic connectivity, with implications for neurodevelopmental disorders.

What is the role of MERTK in the regulation of immune responses, and how does its dysregulation contribute to autoimmune disorders and inflammatory diseases?

MERTK is involved in immune regulation, influencing inflammatory responses. Dysregulation of MERTK has been implicated in autoimmune disorders, emphasizing its role in maintaining immune homeostasis.

What experimental techniques are commonly used to study MERTK, including its ligands, downstream signaling pathways, and cellular functions?

Techniques such as co-immunoprecipitation, flow cytometry, and advanced imaging methods are commonly used to study MERTK, providing insights into ligand interactions, signaling pathways, and cellular functions.