Recombinant Human PON1 293 Cell Lysate

• Cat.No.: PON1-3014HCL

Specification

• Description: Antigen standard for paraoxonase 1 (PON1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PON1 paraoxonase 1 [ Homo sapiens ]
• Official Symbol: PON1
• Synonyms: PON1; paraoxonase 1; PON; serum paraoxonase/arylesterase 1; ESA; esterase A; K-45; PON 1; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; MVCD5
• Gene ID: 5444
• mRNA Refseq: NM_000446
• Protein Refseq: NP_000437
• UniProt ID: P27169
• Chromosome Location: 7q21.3
• Pathway: Metabolic pathways, organism-specific biosystem
• Function: aryldialkylphosphatase activity; arylesterase activity; arylesterase activity; calcium ion binding; hydrolase activity; phospholipid binding; protein homodimerization activity

Reviews

02/06/2022

The manufacturer's team of experts possesses a deep understanding of the PON1 protein and its associated protocols.

07/20/2020

This high-quality PON1 protein provides researchers with confidence in obtaining accurate and reliable data during their investigations

04/27/2019

In conclusion, using PON1 protein in trials offers distinct advantages for researchers studying cell cycle regulation.

Q&As

Are there any genetic tests available to assess PON1 activity?

Yes, genetic tests can determine an individual's PON1 genotype and predict their enzyme activity.

Can PON1 activity be influenced by genetics?

Yes, PON1 activity can be influenced by genetic variations, leading to different enzyme levels and activity.

How is PON1 linked to the metabolism of drugs and xenobiotics?

PON1 can hydrolyze and detoxify certain drugs and environmental toxins, making it important in drug metabolism.

Can PON1 be used to assess an individual's susceptibility to organophosphate toxicity?

Yes, PON1 activity levels can help predict an individual's susceptibility to organophosphate toxicity.

How is PON1 associated with atherosclerosis and heart disease?

PON1 protects against atherosclerosis by preventing the oxidation of LDL cholesterol, a key factor in heart disease.