Recombinant Human PIK3CD, GST-tagged

• Cat.No.: PIK3CD-52H
• Product Overview: Recombinant Human PIK3CD produced inSf9carries a GST-Tag which facilitates the protein's application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110δ chain is 120.2 kDa (without Tag) and the p85α chain 83.5 kDa.

Specification

• Cat. No.: PIK3CD-52H
• Description: The class IA PI3Kδ is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kδ in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgEinduced degranulation and cytokine release. In neutrophils a role for PI3Kδ in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114.
• Source: Sf9 insect cells.
• Physical Appearance: Sterile filtered liquid formualtion.
• Purity: Greater than 95.0% as determined by SDS Page.
• Formulation: 0.5mg/ml solution in 25mM Tris, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.
• Biological Activity: 3 nmol/mg/min using phosphatidylinositol as the substrate.
• Storage: PI3Kb although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide [ Homo sapiens ]
• Synonyms: PIK3CD; phosphoinositide-3-kinase, catalytic, delta polypeptide; PI3K; p110D; P110DELTA; catalytic phosphatidylinositol 3-kinase delta; phosphoinositide-3-kinase C; PI3-kinase p110 subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; EC 2.7.1.153; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PtdIns-3-kinase p110; p110delta; OTTHUMP00000001595
• Gene ID: 5293
• mRNA Refseq: NM_005026
• Protein Refseq: NP_005017
• MIM: 602839
• UniProt ID: O00329
• Chromosome Location: 1p36.2
• Pathway: Acute myeloid leukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Inositol phosphate metabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling system; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; transferase activity