Recombinant Human ACAA1 293 Cell Lysate

• Cat.No.: ACAA1-9119HCL

Specification

• Description: Antigen standard for acetyl-Coenzyme A acyltransferase 1 (ACAA1), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACAA1 acetyl-CoA acyltransferase 1 [ Homo sapiens ]
• Official Symbol: ACAA1
• Synonyms: ACAA1; acetyl-CoA acyltransferase 1; acetyl Coenzyme A acyltransferase 1; 3-ketoacyl-CoA thiolase, peroxisomal; peroxisomal 3 oxoacyl Coenzyme A thiolase; beta-ketothiolase; peroxisomal 3-oxoacyl-CoA thiolase; acetyl-Coenzyme A acyltransferase 1; peroxisomal 3-oxoacyl-Coenzyme A thiolase; ACAA; THIO; PTHIO
• Gene ID: 30
• mRNA Refseq: NM_001130410
• Protein Refseq: NP_001123882
• MIM: 604054
• UniProt ID: P09110
• Chromosome Location: 3p23-p22
• Pathway: Beta-oxidation of very long chain fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: acetyl-CoA C-acyltransferase activity; protein binding; transferase activity, transferring acyl groups other than amino-acyl groups

Reviews

04/22/2022

Provides high signal-to-noise ratio and low background in detection of the target protein in various assays.

11/09/2021

Highly effective in blocking the activity of the target protein in functional studies.

Q&As

What are the potential therapeutic applications of modulating acetyl-CoA acyltransferase 1 protein activity?

Modulating its activity could potentially treat metabolic disorders or enhance ketone body production.

What is the structure of acetyl-CoA acyltransferase 1 protein?

It is a dimeric protein with a helix-turn-helix motif.

What is the role of acetyl-CoA acyltransferase 1 protein in metabolism?

It plays a crucial role in beta-oxidation of fatty acids and ketone body synthesis.

What is the function of acetyl-CoA acyltransferase 1 protein?

It catalyzes the transfer of an acyl group from acyl-CoA to CoA.

How does acetyl-CoA acyltransferase 1 protein interact with other proteins or enzymes?

It interacts with other enzymes in the beta-oxidation pathway, including acyl-CoA dehydrogenases and enoyl-CoA hydratases.

How is the expression of acetyl-CoA acyltransferase 1 protein regulated?

Its expression is regulated by transcription factors, hormones, and metabolic signals.

What are the biochemical pathways that involve acetyl-CoA acyltransferase 1 protein?

It is involved in the metabolism of long-chain fatty acids, branched-chain amino acids, and isoprenoids.

What are the physiological consequences of acetyl-CoA acyltransferase 1 protein deficiency or overexpression?

Deficiency can lead to metabolic disorders, such as hypoketotic hypoglycemia, while overexpression may increase ketone body production.

Where is acetyl-CoA acyltransferase 1 protein expressed in the body?

It is expressed in various tissues, including liver, adipose tissue, and muscle.

How does acetyl-CoA acyltransferase 1 protein contribute to the pathogenesis of metabolic diseases?

Dysregulation of its activity may contribute to the development of metabolic diseases such as diabetes and obesity.