Active Recombinant Human DLK1, His-tagged

• Cat.No.: DLK1-195H
• Product Overview: Recombinant Human DLK1 (Accession # AAA75364) Ala24-Pro297 (Arg248Pro) (Lys295Ser), fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala24 & Phe27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit adipocyte differentiation of mouse 3T3-L1 mouse embryonic fibroblast adipose-like cells. Smas, C.M. et al. (1993) Cell 73:725.3.3 ug/mL of Recombinant Human Pref-1/DLK-1/FA1 will inhibit adipocyte differentiation by more than 50%.
• Molecular Mass: Recombinant Human DLK1, His-tagged has a calculated MW of 29.7 kDa. In SDS-PAGE migrates as 40-55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 250 µg/mL in PBS.
• Publication:
  The Special Role of JAK/STAT, and Notch Signaling Pathways in Cancer Pathogenesis (2023)

Gene Information

• Gene Name: DLK1 delta-like 1 homolog (Drosophila) [ Homo sapiens ]
• Official Symbol: DLK1
• Synonyms: DLK1; delta-like 1 homolog (Drosophila); delta like homolog (Drosophila); protein delta homolog 1; Delta1; FA1; pG2; Pref 1; ZOG; DLK-1; secredeltin; fetal antigen 1; preadipocyte factor 1; DLK; PREF1; Pref-1
• Gene ID: 8788
• mRNA Refseq: NM_003836
• Protein Refseq: NP_003827
• MIM: 176290
• UniProt ID: P80370
• Chromosome Location: 14q32