Active Recombinant Feline CSF2, Met-tagged

• Cat.No.: CSF2-231F
• Product Overview: Recombinant Feline CSF2 (Accession # AAC06041) Ala18-Lys144 (Met36Ile, Thr56Ala & Lys126Asn), fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Feline
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is typically 2 - 8 ng/mL.
• Molecular Mass: Recombinant Feline CSF2, Met-tagged has a calculated MW of 14.6 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Felis catus (domestic cat) ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); GM-CSF; granulocyte-macrophage colony-stimulating factor; CSF; colony-stimulating factor
• Gene ID: 493805
• mRNA Refseq: NM_001009846
• Protein Refseq: NP_001009846
• UniProt ID: O62757