Active Recombinant Human ENPP2, His-tagged

• Cat.No.: ENPP2-3123H
• Product Overview: Recombinant human ENPP2/ATX (ectonucleotide pyrophosphatase-phosphodiesterase 2) is a single polypeptide chain with a 8His tag at the C-terminus. It contains 925 (915+10) amino acids, and having a predicted molecular mass of approximately 106.4kD and migrates in SDS-PAGE with an apparent molecular mass of 110kD.

Specification

• Description: Autotaxin also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) is an enzyme that in humans is encoded by the ENPP2 gene.
• Source: HEK293
• Species: Human
• Tag: His
• Form: 20mM Tris-Cl (pH7.9), 20% Glycerol, 100mM NaCl, 1mM DTT and 0.5mM EDTA
• Bio-activity: ENPP2 protein stimulates the motility of tumor cells with angiogenic properties. It functions as a phosphodiesterase to cleave phosphodiester bonds and a phospholipase to catalyze production of lysophosphatidic acid (LPA). Recombinant human ENPP2 protein is ideal for the studies of its enzyme activity and other related function assays
• AA Sequence: MARRSSFQSCQIISLFTFAVGVNIC LGFTAHRIKRAEGWEEGPPTVLSDS PWTNISGSCKGRCFELQEAGPPDC RCDNLCKSYTSCCHDFDELCLKTAR GWECTKDRCGEVRNEENACHCSEDC LARGDCCTNYQVVCKGESHWVDDD CEEIKAAECPAGFVRPPLIIFSVDG FRASYMKKGSKVMPNIEKLRSCGTH SPYMRPVYPTKTFPNLYTLATGL YPESHGIVGNSMYDPVFDATFHLRG REKFNHRWWGGQPLWITATKQGVKA GTFFWSVVIPHERRILTILQWLTL PDHERPSVYAFYSEQPDFSGHKYGP FGPEESSYGSPFTPAKRPKRKVAPK RRQERPVAPPKKRRRKIHRMDHY AAETRQDKMTNPLREIDKIVGQLMD GLKQLKLHRCVNVIFVGDHGMEDVT CDRTEFLSNYLTNVDDITLVPGTL GRIRSKFSNNAKYDPKAIIANLTCK KPDQHFKPYLKQHLPKRLHYANNRR IEDIHLLVERRWHVARKPLDVYK KPSGKCFFQGDHGFDNKVNSMQTVF VGYGSTFKYKTKVPPFENIELYNVM CDLLGLKPAPNNGTHGSLNHLLRT NTFRPTMPEEVTRPNYPGIMYLQSD FDLGCTCDDKVEPKNKLDELNKRLH TKGSTEERHLLYGRPAVLYRTRY DILYHTDFESGYSEIFLMPLWTSYT VSKQAEVSSVPDHLTSCVRPDVRVS PSFSQNCLAYKNDKQMSYGFLFPP YLSSSPEAKYDAFLVTNMVPMYPAF KRVWNYFQRVLVKKYASERNGVNVI SGPIFDYDYDGLHDTEDKIKQYV EGSSIPVPTHYYSIITSCLDFTQPA DKCDGPLSVSSFILPHRPDNEESCN SSEDESKWVEELMKMHTARVRDIE HLTSLDFFRKTSRSYPEILTLKTYL HTYESEIGGHHHHHHHH
• Purity: ≥90%, as determined by SDS-PAGE
• Usage: FOR RESEARCH ONLY
• Storage: The protein sample can be stored under sterile conditions at 2- 8 centigrade for one month or at -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 [ Homo sapiens ]
• Official Symbol: ENPP2
• Synonyms: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D
• Gene ID: 5168
• mRNA Refseq: NM_001040092
• Protein Refseq: NP_001035181
• MIM: 601060
• UniProt ID: Q13822
• Chromosome Location: 8q24.1
• Pathway: Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem
• Function: alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity