Recombinant Human MMP1 precursor pro form (NP_002412.1) (Met 1-Asn 469), fused with a C-terminal polyhistidine tag, was produced in Human Cell.
Lyophilized from sterile 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 5.5
The secreted recombinant human MMP1 comprises 461 amino acids migrates as an approximately 50-55 kDa band as estimated in SDS-PAGE under reducing conditions.
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.