Recombinant Human IL34 fused with His tag at C-terminal was expressed in HEK293.
IL-34 is a homodimeric cytokine that is expressed in a range of tissues including spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1 stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1.
Measured by its ability to induce secretion of MCP-1 from PBMCs.
460 amino acid residues
NEPLEMWPL TQNEECTVT GFLRDKLQY RSRLQYMKH YFPINYKIS VPYEGVFRI ANVTRLQRA QVSERELRY LWVLVSLSA TESVQDVLL EGHPSWKYL QEVETLLLN VQQGLTDVE VSPKVESVL SLLNAPGPN LKLVRPKAL LDNCFRVME LLYCSCCKQ SSVLNWQDC EVPSPQSCS PEPSLQYAA TQLYPPPPW SPSSPPHST GSVRPVRAQ GEGLLPHHH HHHHH
Above 95% as determined by SDS-PAGE Analysis.