Recombinant Human CDC-like Kinase 1, GST-tagged

• Cat.No.: CLK1-676H
• Product Overview: Recombinant human CDC-Like Kinase 1 catalytic domain (aa 129-484) with GST tag was expressed inE. coli.MW = 70.1 kDa.

Specification

• Cat. No.: CLK1-676H
• Description: CLK/STY is a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. The phosphorylated serine/arginine-rich (SR) proteins involves in the pre-mRNA processing and releasing through nucleus into nucleoplasm. CLK/STY, which could phosphorylates the specific SR proteins, such as ASF/SF2, may directly regulates the activity and compartmentalization of SR splicing factors.
• Source: E. coli.
• Formulation: Liquid in 50 mM Tris, pH 7.5 + 150 mM NaCl + 0.05 mM EDTA + 0.05% Triton X-100 + 2mM DTT + 50% glycerol.
• Specific Activity: 79 nmole of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33 µg/ml.
• Storage & Stability: Stable for 6 months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CLK1 CDC-like kinase 1 [ Homo sapiens ]
• Synonyms: CLK1; CDC-like kinase 1; CLK; STY; CLK/STY; CDC28/CDC2-like kinase; protein tyrosine kinase STY; dual specificity protein kinase CLK1; EC 2.7.12.1
• Gene ID: 1195
• mRNA Refseq: NM_001162407
• Protein Refseq: NP_001155879
• MIM: 601951
• UniProt ID: P49759
• Chromosome Location: 2q33
• Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity

Reviews

01/31/2021

The manufacturer's commitment to customer satisfaction extends beyond technical support. They offer a comprehensive range of related products and resources to aid researchers in their trials.

01/22/2019

From troubleshooting to optimizing experimental protocols, the manufacturer provides diligent support to ensure the success of the trial.

09/28/2018

The CLK1 protein offers several advantageous features that make it an ideal choice for researchers conducting trials.

Q&As

How is CLK1 expression regulated in normal physiological conditions?

CLK1 expression is tightly regulated at transcriptional and post-translational levels, ensuring its proper function in normal cellular processes.

How is CLK1 activity measured in research settings?

Researchers often measure CLK1 activity through in vitro kinase assays or by monitoring its phosphorylation targets in cellular or tissue samples.

Can targeting CLK1 have implications for neurodegenerative diseases?

Yes, studies suggest that CLK1 inhibitors may have therapeutic potential for neurodegenerative disorders by modulating RNA splicing.

Are there any known CLK1 mutations associated with diseases?

Some studies suggest that CLK1 mutations may be associated with certain diseases, but further research is needed to establish clear links.

Are there any CLK1 inhibitors under development for cancer treatment?

Yes, several CLK1 inhibitors are in preclinical and clinical development as potential anticancer agents.