Active Recombinant Mouse Hpx protein, His-tagged

• Cat.No.: Hpx-3282M
• Product Overview: Recombinant Mouse Hpx protein(NP_059067.2)(Met1-Gln460), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant mouse Hemopexin binds >15 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of mouse Hemopexin.
• Molecular Mass: The recombinant mouse HPX consists of 448 amino acids and has a predicted molecular mass of 50.4 kDa. The apparent molecular mass of the rm HPX is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Proteinlength: Met1-Gln460
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Hpx hemopexin [ Mus musculus ]
• Official Symbol: Hpx
• Synonyms: HPX; hemopexin; hx; Hpxn
• Gene ID: 15458
• mRNA Refseq: NM_017371
• Protein Refseq: NP_059067