Recombinant Human ACE2, FLAG-tagged

Cat. No.

ACE2-735H

Product Overview

Recombinant human ACE-2 (amino acid Met1~Ser740) encoding the original signal peptide sequence and the extracellular domain was tagged with FLAG (DYKDDDDK) at the C-terminus. The tagged protein was expressed in HEK-293 and purified from c.

Description

Angiotensin I converting enzyme 2 (ACE2) is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin,or the conversion of angiotensin II to angiotensin 1-7. ACE 2 has direct effects on cardiac functiona, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.

Source

HEK-293.

Purity

>90%, as determined by SDS-PAGE.

Form

0.2 m filtered solution in phosphate-buffered saline.

Activity

Hydrolysis of a fluorescent peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH.

Endotoxin Level

<0.1EU/mg, as determined by the LAL method.

Storage

The soluble proteins should be stored in working aliquots at -20oC for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]

Synonyms

ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; ACEH; DKFZp434A014; angiotensin I converting enzyme; ACE-related carboxypeptidase2; angiotensin converting enzyme-like protein; EC 3.4.15.1; EC 3.4.17.-; Angiotensin-converting enzyme 2; ACEH

GeneID

59272

mRNA Refseq

NM_021804

Protein Refseq

NP_068576

MIM

300335

UniProt ID

Q9BYF1

Chromosome Location

Xp22

Pathway

Renin-angiotensin system

Function

carboxypeptidase activity; chloride ion binding; glycoprotein binding; metal ion binding; metallopeptidase activity; peptidase activity; peptidyl-dipeptidase activity; viral receptor activity; zinc ion binding