GMP Recombinant Human DKK1 Protein

Cat.No.: DKK1-145HG
Product Overview: Recombinant Human DKK1, Thr32-His266, was produced in Sf 21 (baculovirus)-derived under cGMP guidelines.
Source: Insect cell
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
Molecular Mass: Predicted Molecular Mass: 25.8 kDa
SDS-PAGE: 33-38 kDa, reducing conditions
N-terminal Sequence Analysis: Thr-Leu-Asn-Ser-Val-Leu-Asn-Ser-Asn-Ala
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE with silver staining.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
Official Symbol: DKK1
Synonyms: DKK1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1;
Gene ID: 22943
mRNA Refseq: NM_012242
Protein Refseq: NP_036374
MIM: 605189
UniProt ID: O94907

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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