"IL12B" Related Products

GMP Recombinant Human IL12B Protein

Cat.No.: IL12B-128HG
Product Overview: Recombinant Human IL12B(Ile23–Ser328) and Human IL12A(Arg23–Ser219), was co-produced in Sf 21 (baculovirus)-derived under cGMP guidelines.
Source: Insect cell
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
Molecular Mass: Predicted Molecular Mass: 34.7 (p40) & 22.5 (p35) kDa
SDS-PAGE: 41 kDa (p40) and 29 kDa (p35), reducing conditions
N-terminal Sequence Analysis: Ile-Trp-Glu-Leu-Lys-Lys-Asp-Val-Tyr-Val (p40)
Arg-Asn-Leu-Pro-Val-Ala-Thr-Pro-Asp-Pro (p35)
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
Official Symbol: IL12B
Synonyms: IL12B; NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; IL-12B;
Gene ID: 3593
mRNA Refseq: NM_002187
Protein Refseq: NP_002178
MIM: 161561
UniProt ID: P29460

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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