Active GMP Recombinant Human IL19 protein
Cat.No. : | IL19-4332HG |
Product Overview : | Recombinant Human IL19 was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines. |
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Description : | Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 × 10^5 IU/mg. |
Molecular Mass : | Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
AA Sequence : | LRRCLISTDMHHIEESFQEIKRAIQ AKDTFPNVTILSTLETLQIIKPLDV CCVTKNLLAFYVDRVFKDHQEPNPK ILRKISSIANSFLYMQKTLRQCQEQ RQCHCRQEATNATRVIHDNYDQLEV HAAAIKSLGELDVFLAWINKNHEVM SSA |
Endotoxin : | Less than 1 EU/µg of rHuIL-19 as determined by LAL method. |
Purity : | > 95 % by SDS-PAGE and HPLC analyses. |
Storage : | This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Reconstitution : | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Gene Name : | IL19 interleukin 19 [ Homo sapiens (human) ] |
Official Symbol : | IL19 |
Synonyms : | MDA1; NG.1; ZMDA1; IL-10C; Melanoma Differentiation-associated Protein-like Protein, NG.1 |
Gene ID : | 29949 |
mRNA Refseq : | NM_153758.2 |
Protein Refseq : | NP_715639.1 |
MIM : | 605687 |
UniProt ID : | Q9UHD0 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionConfocal microscopy and flow cytometry visualize IL19-induced cellular responses and track receptor internalization upon ligand binding.
Functional assays using immune cell cultures and cytokine profiling assays dissect IL19's impact on immune cell polarization and cytokine secretion.
Biophysical techniques like surface plasmon resonance and analytical ultracentrifugation quantify IL19's binding to IL20RA and IL20RB and reveal receptor complex composition.
Biophysical techniques such as surface plasmon resonance and isothermal titration calorimetry quantify IL19's binding kinetics to its receptor complexes.
CRISPR/Cas9 knockout models and siRNA-mediated knockdown in specific cell types unravel IL19's cell-specific roles.
Neutralizing antibodies, receptor blockade experiments, and in vivo disease models evaluate IL19's suitability as a therapeutic target in inflammatory and autoimmune diseases.
Recombinant protein expression in bacterial or mammalian systems followed by purification allows generation of functional IL19 for various experimental assays.
Reporter gene assays, ChIP, and DNA-protein interaction studies probe IL19's transcriptional regulation and interactions with specific DNA sequences.
Animal models and patient samples, combined with histopathology and molecular assays, shed light on IL19's involvement in skin disorders and its therapeutic potential.
Animal wound healing models, in vitro cell migration assays, and co-culture experiments investigate IL19's role in tissue repair and its effects on fibroblast and keratinocyte function.
Customer Reviews (3)
Write a reviewHigh reproducibility.
Suitable for Western blot.
Effective in cell differentiation.
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