Intracellular Nitric Oxide Synthase (NOS) Detection Kit

• Cat.No.: Kit-2404
• Product Overview: Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved with the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. Intracellular Nitric Oxide Synthase Detection kit uses a dye that reacts with intracellular NO produced by NOS to produce fluorescence (Ex/Em = 485/530 nm), which is proportional to the concentration of intracellular NOS and can be detected using a microplate reader or a fluorescence microscope. This kit provides a simple, non-radiometric method for detection of intracellular NOS in the cells.

Specification

• Applications: Detection of NOS activity in adherent cells.
  Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of NOS.
• Storage: -20°C
• Size: 100 assays

Reviews

02/18/2023

The NOS proteins purchased from your company is suitable for ELISA.

03/12/2022

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

12/01/2020

Cheap price, high quality products, worth buying again.

Q&As

In which cells is NOS present?

NOS are present in neurophagocytes, endothelial cells, macrophages.

Can NOS add tags other than his?

Of course, we will provide you with the most suitable tags according to your needs.

NOS is closely related to the study of which diseases?

NOS is associated with abnormal vascular endothelial function, cerebral infarction and other processes.

What is the effect of lipid-lowering drugs to NOS expression?

Lipid-lowering drugs can increase the expression of NOS.