Recombiant Hmuan AlkB, Alkylation Repair Homolog 2, His-tagged
Cat.No. : | ALKBH2-977H |
Product Overview : | Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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Cat. No. : | ALKBH2-977H |
Description : | Alpha-ketoglutarate-dependent dioxygenasealkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and α-ketoglutarate for enzymatic activity. |
Formulation : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol |
Molecular Weight : | 31.4kDa (281aa), confirmed by MALDI-TOF |
Purity : | > 90% by SDS – PAGE |
Sequences of amino acids : | MGSSHHHHH HSSGLVPRGSHMDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K |
concentration : | 0.5 mg/ml (determined by Bradford assay) |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. Avoid repeated freezing and thawing cycles. |
Gene Name : | ALKBH2 alkB, alkylation repair homolog 2 (E. coli) [ Homo sapiens ] |
Synonyms : | ALKBH2;alkB, alkylation repair homolog 2 (E. coli);ABH2; FLJ99103; MGC90512; alpha-ketoglutarate-dependent dioxygenasealkB homolog 2;oxy DC1;2OG-Fe(II) oxy DC1;alkylated DNA repair protein alkB homolog 2;EC 1.14.11. |
Gene ID : | 121642 |
MIM : | 610602 |
mRNA Refseq : | NM_001001655 |
Protein Refseq : | NP_001001655 |
UniProt ID : | Q6NS38 |
Chromosome Location : | 12q24.11 |
Function : | 2-oxoglutarate-dependent dioxygenase activity;DNA-N1-methyladenine dioxygenase activity;cytosine C-5 DNA demethylase activity;damaged DNA binding;ferrous iron binding;metal ion binding;oxidoreductase activity;oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen. |
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◆ Lysates | ||
ALKBH2-8902HCL | Recombinant Human ALKBH2 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (18)
Ask a questionThe functional regulation of ALKBH2 may be affected by epigenetic changes, such as histone modification and DNA methylation.
Mutations in ALKBH2 may affect the development of embryos and lead to related diseases.
ALKBH2 is involved in biological processes such as double-stranded DNA repair and cell cycle control.
ALKBH2 plays an important role in the DNA demethylation pathway and is involved in a number of DNA repair related pathways.
ALKBH2 interacts with a series of DNA repair related proteins such as DNA methyltransferase and DNA repair activator to participate in DNA repair.
ALKBH2 gene contains 10 exons and its expression is influenced by several regulatory elements and signaling pathways.
The activity of ALKBH2 may be regulated and interfered with by some chemicals, but the specific mechanism of action needs further study.
It is mainly used to modify DNA, but it also has some ability to modify RNA.
Overexpression or knockout of ALKBH2 can lead to incomplete repair of DNA damage and affect cell proliferation and division.
ALKBH2 is mainly found in the nucleus and is involved in biological processes such as DNA repair.
ALKBH2 removes methyl groups from DNA bases and is involved in DNA repair and cell proliferation.
This protein plays an important role in a variety of tumors, such as colorectal cancer, small cell lung cancer and so on.
A number of inhibitors have been developed that target ALKBH2 and inhibit its ability to acquire DNA methylation repair.
It is involved in multiple DNA single and double strand damage repair pathways, including light damage repair pathways.
Current research data show that ALKBH2 is associated with gene transcription, but the specific mechanism of action remains to be explored.
Mutations in ALKBH2 have been linked to some DNA repair related diseases and cancers.
It plays an important role in several neurological diseases, such as neurodegenerative diseases and Parkinson's disease.
ALKBH2 is involved in biological processes such as DNA repair, cell proliferation and differentiation.
Customer Reviews (5)
Write a reviewExhibited the expected biological activities, such as enzymatic activity and receptor binding activity.
ALKBH2 is highly expressed and can efficiently synthesize a large number of target proteins in host cells.
Good crystallization properties when analyzed by X-ray crystallography or other crystallization techniques.
The products are reliable and stable, and are widely used in scientific research, industrial production and medical fields.
The purity of ALKBH2 was good and the results obtained after analysis by SDS-PAGE or Western blot were excellent.
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