| Description : |
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His(N-ter) |
| Form : |
Powder |
| Bio-activity : |
Determined by its ability to induce proliferation in FDC-P1 cells. The ED50 for this effect is < 50 pg/ml. The specific activity of recombinant mouse GM-CSF is approximately > 2x 10^7 IU/mg. |
| AA Sequence : |
APTRSPITVTRPWKHVEAIKEALNL LDDMPVTLNEEVEVVSNEFSFKKLT CVQTRLKIFEQGLRGNFTKLKGALN MTASYYQTYCPPTPETDCETQVTTY ADFIDSLKTFLTDIPFECKKPVQK |
| Endotoxin : |
Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test. |
| Purity : |
> 98% (by SDS-PAGE) |
| Applications : |
SDS-PAGE |
| Notes : |
For laboratory research only, not for drug, diagnostic or other use. |
| Storage : |
Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Storage Buffer : |
PBS (pH 7.4) |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
