Recombinant Cynomolgus ALPP protein, His-tagged

• Cat.No.: ALPP-2860C
• Product Overview: Recombinant Cynomolgus ALPP protein(Ile 21-Asn 504), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Supplied as 0.2 μm filtered solution in 50mM Tris,150mM NaCl pH7.3 with trehalose as protectant.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 54.6 kDa. The protein migrates as 61-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Ile 21-Asn 504
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

12/01/2020

High-throughput screening compatibility.

11/05/2018

Consistent protein expression analysis.

10/25/2017

Precise protein quantification in ELISA.

Q&As

Can ALPP serve as a diagnostic biomarker or therapeutic target for specific diseases?

ALPP may serve as a diagnostic biomarker for specific diseases, allowing for disease detection and monitoring, and its targeting could hold therapeutic potential in certain pathological conditions.

How does ALPP contribute to the metabolism and turnover of its substrate(s) in physiological and pathological conditions?

ALPP participates in the metabolism and turnover of its substrate(s), including the hydrolysis of phosphoester bonds and the regulation of cellular phosphate homeostasis.

What are the downstream signaling pathways and molecular interactions regulated by ALPP in cellular functions?

ALPP regulates downstream signaling pathways and molecular interactions involved in cellular processes like phosphate metabolism, intracellular signaling, and extracellular matrix remodeling.

What are the regulatory mechanisms controlling ALPP gene expression and enzyme activity?

ALPP gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in cellular homeostasis and disease processes.

What is the subcellular localization and trafficking of ALPP within cells?

ALPP is localized to specific cellular compartments, such as the plasma membrane, endoplasmic reticulum, or lysosomes, and its trafficking may be regulated by sorting signals and intracellular transport machinery.

Can ALPP activity be modulated by specific factors or inhibitors, and how does this impact cellular processes?

ALPP activity can be modulated by factors such as pH, calcium, and inhibitors, which can affect its catalytic efficiency and cellular functions.

How does ALPP deficiency or dysregulation contribute to the pathogenesis of diseases and disorders?

ALPP deficiency or dysregulation is associated with various diseases, including metabolic disorders, bone diseases, and certain cancers, highlighting its pathological implications.

How can ALPP activity and function be manipulated to modulate cellular processes and disease outcomes?

Manipulating ALPP activity and function could offer strategies to modulate cellular processes and disease outcomes, potentially leading to therapeutic interventions and improved patient outcomes.

How does ALPP expression and activity change during development, differentiation, and disease progression?

ALPP expression and activity exhibit dynamic changes during development, cellular differentiation, and disease progression, reflecting its roles in different physiological and pathological contexts.

What is the expression pattern of ALPP in different tissues and cell types?

ALPP shows a tissue-specific expression pattern, with higher levels in tissues such as liver, bone, and kidney, and lower levels in other organs.