Recombinant E. coli BirA protein(Met1-Lys321), His&MBP-tagged

• Cat.No.: BirA-3247E
• Product Overview: Recombinant E.coil BirA (P06709) (Met1–Lys321) was expressed in E. coli, fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

Specification

• Source: E. coli
• Species: E. coli
• Tag: N-His&MBP
• Protein length: Met1-Lys321
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.