Recombinant Escherichia coli O157:H7 birA Protein (M1-K321), His-tagged

• Cat.No.: birA-1439E
• Product Overview: Recombinant Escherichia coli O157:H7 N-6His-BirA protein with a His tag was expressed in E. coli

Specification

• Description: Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
• Source: E. coli
• Species: Escherichia coli O157:H7
• Tag: His
• Form: Liquid
• Protein Length: M1-K321
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: birA bifunctional protein BirA [ Escherichia coli O157:H7 str. Sakai ]
• Official Symbol: birA
• Synonyms: birA; bifunctional protein BirA; bifunctional protein BirA
• Gene ID: 914965
• Protein Refseq: NP_312927
• UniProt ID: Q8X709