Recombinant Full Length Chicken Insulin-Induced Gene 2 Protein(Insig2) Protein, His-Tagged

• Cat.No.: RFL6710GF
• Product Overview: Recombinant Full Length Chicken Insulin-induced gene 2 protein(INSIG2) Protein (Q5F3W2) (1-167aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Gallus gallus (Chicken)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-167)
• AA Sequence: MAENDAKPTLPKKSGPYISSVTSRG MNLVIRGIVLFFIGVFLALVLNLLN AVCSGLCQGI NHASAKVDFANNIQLSLTLAALSIG LWWTFDRSRSGFGLGVGIAFLATLV SQLLVYNGVY QYTSPDFIYVRSWLPCIFFAGGITM GNIGRQLAMYECKVIAEKSHED
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: INSIG2
• Synonyms: INSIG2; RCJMB04_5j21; Insulin-induced gene 2 protein; INSIG-2
• UniProt ID: Q5F3W2

Gene Information

• Gene Name: INSIG2
• Synonyms: INSIG2; RCJMB04_5j21; Insulin-induced gene 2 protein; INSIG-2
• UniProt ID: Q5F3W2

Reviews

08/16/2020

We observed that results obtained with the product remained stable and reliable over long-term experiments.

01/11/2020

The product's impact extended to data analysis, with intuitive tools for interpreting experimental results.

07/07/2017

Its adaptability to various sample types and conditions made it a valuable asset for our diverse projects.

Q&As

Are there any post-translational modifications known to regulate INSIG2?

While specific post-translational modifications may exist, the current literature provides limited information on post-translational regulation of INSIG2.

How does INSIG2 contribute to insulin sensitivity?

INSIG2 contributes to insulin sensitivity by regulating lipid metabolism and insulin signaling pathways.

How does INSIG2 impact the activity of SREBP-1 and SREBP-2?

INSIG2 inhibits the activity of both SREBP-1 and SREBP-2, impacting lipogenesis and cholesterol biosynthesis.

What is the relationship between INSIG2 and cholesterol synthesis?

INSIG2 is a key regulator of cholesterol synthesis, modulating the activation of SREBP-2, a master regulator of cholesterol biosynthesis.

What is the role of INSIG2 in cellular response to ER stress?

INSIG2 is involved in the cellular response to ER stress, playing a role in maintaining ER homeostasis.

Is there evidence of INSIG2 involvement in cancer development?

There is evidence linking INSIG2 to cancer development, suggesting a potential role in tumorigenesis.

Does INSIG2 expression vary in response to different dietary conditions?

Yes, INSIG2 expression can vary in response to different dietary conditions, reflecting its role in metabolic adaptation.