Recombinant Full Length Human ACSF3 Protein, GST-tagged

• Cat.No.: ACSF3-788HF
• Product Overview: Human ACSF3 full-length ORF ( AAH28399.1, 1 a.a. - 576 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 90.6 kDa
• Protein Length: 576 amino acids
• AA Sequence: MPPHVVLTFR RLGCALASCR LAPARHRGSG LLHTAPVARS DRSAPVFTRA LAFGDRIALV DQHGRHTYRE LYSRSLRLSQ EICRLCGCVG GDLREERVSF LCANDASYVV AQWASWMSGG VAVPLYRKHP AAQLEYVICD SQSSVVLVSQ EYLELLSPVV RKLGVPLLPL TPAIYTGAVE EPAEVPVPEQ GWRNKGAMII YTSGTTGRPK GVLSTHQNIR AVVTGLVHKW AWTKDDVILH VLPLHHVHGV VNALLCPLWV GATCVMMPEF SPQQVWEKFL SSETPRINVF MAVPTIYTKL MEYYDRHFTQ PHAQDFLRAV CEEKIRLMVS GSAALPLPVL EKWKNITGHT LLERYGMTEI GMALSGPLTT AMRLPGSVGT PLPGVQVRIV SENPQREACS YTIHAEGDER GTKVTPGFEE KEGELLVRGP SVFREYWNKP EETKSAFTLD GWFKTGDTVV FKDGQYWIRG RTSVDIIKTG GYKVSALEVE WHLLAHPSIT DVAVIGVPDM TWGQRVTAVV TLREGHSLSH RELKEWARNV LAPYAVPSEL VLVEEIPRNQ MGKIDKKALI RHFHPS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACSF3 acyl-CoA synthetase family member 3 [ Homo sapiens ]
• Official Symbol: ACSF3
• Synonyms: ACSF3; acyl-CoA synthetase family member 3; acyl-CoA synthetase family member 3, mitochondrial; FLJ39242
• Gene ID: 197322
• mRNA Refseq: NM_001127214
• Protein Refseq: NP_001120686
• MIM: 614245
• UniProt ID: Q4G176

Reviews

11/05/2022

This is my go-to supplier for recombinant proteins. Always reliable and consistent.

09/10/2021

The purity of the recombinant protein was exceptional. Very impressed.

01/20/2019

Highly recommend this company for all your recombinant protein needs.

05/05/2018

Quick and easy ordering process. Received my recombinant protein in a timely manner.

01/19/2018

The recombinant protein arrived on time and in perfect condition. No issues at all.

Q&As

What is the relationship between ACSF3 and obesity?

Several studies have found a correlation between ACSF3 gene expression and obesity, with higher ACSF3 expression levels observed in obese individuals.

What is the biochemical function of ACSF3 in phospholipid synthesis?

ACSF3 catalyzes the activation of fatty acids for use in phospholipid synthesis, a crucial component of membrane structure and function.

What is the role of ACSF3 in the production of the neurotransmitter acetylcholine?

ACSF3 is involved in the production of acetyl-CoA, which is a substrate for the enzyme choline acetyltransferase (ChAT) that catalyzes the formation of acetylcholine from choline and acetyl-CoA. Therefore, ACSF3 indirectly contributes to the production of acetylcholine, which is an important neurotransmitter in the nervous system.

What is the mechanism of ACSF3 in fatty acid activation?

ACSF3 catalyzes the activation of long-chain fatty acids by transferring the fatty acid to CoA, forming acyl-CoA, which can be used in various metabolic processes.

How does ACSF3 enzyme interact with other proteins?

ACSF3 interacts with several proteins, including fatty acid transport proteins, diacylglycerol acyltransferases, and peroxisome proliferator-activated receptors.

What are the implications of ACSF3 dysregulation?

Dysregulation of ACSF3 gene expression has been linked to several metabolic disorders, including obesity, insulin resistance, and type 2 diabetes.

What is the role of ACSF3 in the metabolism of branched-chain fatty acids?

ACSF3 has been shown to activate branched-chain fatty acids, such as phytanic acid and pristanic acid, which are derived from dietary sources or produced by gut microbiota. ACSF3 activates these fatty acids by converting them into their respective CoA esters, which can then be further metabolized in the peroxisomes.

How is ACSF3 gene expression regulated?

ACSF3 gene expression is regulated by various factors, including hormones, transcription factors, and epigenetic modifications.

How is ACSF3 expression regulated in cells?

The expression of ACSF3 can be regulated at both the transcriptional and post-transcriptional levels. Transcriptional regulation can be mediated by factors such as peroxisome proliferator-activated receptor (PPAR) and sterol regulatory element-binding protein (SREBP), which can activate or repress the transcription of the ACSF3 gene. Post-transcriptional regulation can occur through mechanisms such as alternative splicing, microRNA-mediated degradation, and protein degradation via the ubiquitin-proteasome system.

What is the subcellular localization of ACSF3?

ACSF3 is primarily localized in the mitochondria of cells, specifically in the mitochondrial outer membrane, where it functions in the beta-oxidation of fatty acids.