Recombinant Full Length Human ACSL3 Protein, C-Flag-tagged

• Cat.No.: ACSL3-1459HFL
• Product Overview: Recombinant Full Length Human ACSL3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 80.2 kDa
• AA Sequence: MNNHVSSKPSTMKLKHTINPILLYF IHFLISLYTILTYIPFYFFSESRQE KSNRIKAKPVNSKPDSAYRS VNSLDGLASVLYPGCDTLDKVFTYA KNKFKNKRLLGTREVLNEEDEVQPN GKIFKKVILGQYNWLSYEDV FVRAFNFGNGLQMLGQKPKTNIAIF CETRAEWMIAAQACFMYNFQLVTLY ATLGGPAIVHALNETEVTNI ITSKELLQTKLKDIVSLVPRLRHII TVDGKPPTWSEFPKGIIVHTMAAVE ALGAKASMENQPHSKPLPSD IAVIMYTSGSTGLPKGVMISHSNII AGITGMAERIPELGEEDVYIGYLPL AHVLELSAELVCLSHGCRIG YSSPQTLADQSSKIKKGSKGDTSML KPTLMAAVPEIMDRIYKNVMNKVSE MSSFQRNLFILAYNYKMEQI SKGRNTPLCDSFVFRKVRSLLGGNI RLLLCGGAPLSATTQRFMNICFCCP VGQGYGLTESAGAGTISEVW DYNTGRVGAPLVCCEIKLKNWEEGG YFNTDKPHPRGEILIGGQSVTMGYY KNEAKTKADFFEDENGQRWL CTGDIGEFEPDGCLKIIDRKKDLVK LQAGEYVSLGKVEAALKNLPLVDNI CAYANSYHSYVIGFVVPNQK ELTELARKKGLKGTWEELCNSCEME NEVLKVLSEAAISASLEKFEIPVKI RLSPEPWTPETGLVTDAFKL
  KRKELKTHYQADIERMYGRKTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway

Gene Information

• Gene Name: ACSL3 acyl-CoA synthetase long chain family member 3 [ Homo sapiens (human) ]
• Official Symbol: ACSL3
• Synonyms: ACS3; FACL3; LACS3; LACS 3; PRO2194
• Gene ID: 2181
• mRNA Refseq: NM_004457.5
• Protein Refseq: NP_004448.2
• MIM: 602371
• UniProt ID: O95573

Reviews

06/14/2021

The product was well-characterized, and the documentation provided was thorough and helpful.

08/27/2020

The protein arrived quickly and was exactly what I needed for my experiment. Very satisfied with my purchase.

06/01/2020

The website was easy to navigate and the ordering process was simple. Appreciate the user-friendly interface.

10/04/2019

I'm really pleased with my purchase of the recombinant protein. It's high quality and reasonably priced.

09/01/2018

I appreciate the transparency and honesty of this company in their communication with customers. Will continue to order from them in the future.

Q&As

What is the relationship between ACSL3 and cardiovascular disease?

ACSL3 dysregulation has been linked to the development of cardiovascular disease, highlighting the importance of the gene in the regulation of lipid metabolism and cardiovascular health.

What is the impact of ACSL3 on autophagy?

ACSL3 has been shown to impact autophagy, a cellular process involved in the breakdown and recycling of damaged or dysfunctional cellular components.

What is the relationship between ACSL3 and insulin resistance?

ACSL3 dysregulation has been implicated in the development of insulin resistance, a common feature of many metabolic disorders.

What is the impact of ACSL3 on adipogenesis?

ACSL3 has been shown to impact adipogenesis, the process of fat cell differentiation, by regulating the availability of long-chain fatty acids for lipid synthesis and storage.

What is the structure of the ACSL3 protein?

The ACSL3 protein consists of multiple domains, including an N-terminal domain, a central domain, and a C-terminal domain, which are involved in substrate binding, acyl-adenylate formation, and ATP binding, respectively.

What is the impact of ACSL3 on cellular signaling pathways?

ACSL3 has been shown to impact cellular signaling pathways, particularly those involved in the regulation of inflammation, insulin signaling, and energy metabolism.

What is the role of ACSL3 in lipid metabolism?

ACSL3 plays a critical role in lipid metabolism by activating long-chain fatty acids for subsequent utilization in various metabolic pathways, including β-oxidation and the production of signaling lipids.

What is the expression pattern of ACSL3 in different tissues?

ACSL3 is expressed in a variety of tissues, including adipose tissue, liver, muscle, heart, and brain, with highest expression levels observed in adipose tissue.

How does ACSL3 impact lipid droplet formation?

ACSL3 has been shown to impact lipid droplet formation, with increased expression of the gene leading to enhanced lipid droplet formation and storage.

How does ACSL3 impact cellular proliferation and differentiation?

ACSL3 has been shown to impact cellular proliferation and differentiation by modulating the availability of long-chain fatty acids for various cellular processes.