Recombinant Full Length Human ACSS1 Protein, C-Flag-tagged

• Cat.No.: ACSS1-1737HFL
• Product Overview: Recombinant Full Length Human ACSS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 71.1 kDa
• AA Sequence: MAARTLGRGVGRLLGSLRGLSGQPA RPPCGVSAPRRAASGPSGSAPAVAA AAAQPGSYPALSAQAAREPA AFWGPLARDTLVWDTPYHTVWDCDF STGKIGWFLGGQLNVSVNCLDQHVR KSPESVALIWERDEPGTEVR ITYRELLETTCRLANTLKRHGVHRG DRVAIYMPVSPLAVAAMLACARIGA VHTVIFAGFSAESLAGRIND AKCKVVITFNQGLRGGRVVELKKIV DEAVKHCPTVQHVLVAHRTDNKVHM GDLDVPLEQEMAKEDPVCAP ESMGSEDMLFMLYTSGSTGMPKGIV HTQAGYLLYAALTHKLVFDHQPGDI FGCVADIGWITGHSYVVYGP LCNGATSVLFESTPVYPNAGRYWET VERLKINQFYGAPTAVRLLLKYGDA WVKKYDRSSLRTLGSVGEPI NCEAWEWLHRVVGDSRCTLVDTWWQ TETGGICIAPRPSEEGAEILPAMAM RPFFGIVPVLMDEKGSVMEG SNVSGALCISQAWPGMARTIYGDHQ RFVDAYFKAYPGYYFTGDGAYRTEG GYYQITGRMDDVINISGHRL GTAEIEDAIADHPAVPESAVIGYPH DIKGEAAFAFIVVKDSAGDSDVVVQ ELKSMVATKIAKYAVPDEIL
  VVKRLPKTRSGKVMRRLLRKIITSE AQELGDTTTLEDPSIIAEILSVYQK CKDKQAAAKTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism

Gene Information

• Gene Name: ACSS1 acyl-CoA synthetase short chain family member 1 [ Homo sapiens (human) ]
• Official Symbol: ACSS1
• Synonyms: ACAS2L; ACECS1; AceCS2L
• Gene ID: 84532
• mRNA Refseq: NM_032501.4
• Protein Refseq: NP_115890.2
• MIM: 614355
• UniProt ID: Q9NUB1

Reviews

09/17/2022

Wow, this protein is like a superhero in our lab! It saves us time and effort while delivering top-notch performance.

06/01/2022

We've been using this protein for protein-protein interaction studies, and it has exceeded our expectations. It exhibits strong and specific binding with our target.

10/15/2021

Exceptional customer service that ensures a smooth experience. Quality protein that delivers results.

08/14/2019

This product has significantly accelerated our research progress. Its high activity and excellent functionality have allowed us to obtain meaningful data faster.

05/13/2018

I'm just blown away by the awesomeness of this protein! It's like having a trusted lab buddy that never lets you down. Love it!

Q&As

What is the mechanism of regulation of ACSS1 protein expression?

ACSS1 protein expression is regulated by a variety of factors including nutrient availability, hypoxia, and oncogenic signaling pathways.

What is the role of ACSS1 protein in the regulation of inflammation?

ACSS1 protein has been implicated in the regulation of inflammatory gene expression and the production of pro-inflammatory cytokines.

What is the role of ACSS1 protein in the regulation of gene expression?

ACSS1 protein is involved in the acetylation of histones, which can regulate gene expression by modulating chromatin structure.

What is the effect of ACSS1 protein inhibition on cancer cell growth?

Inhibition of ACSS1 protein activity has been shown to impair cancer cell growth and induce cell death in vitro and in vivo.

What is the relationship between ACSS1 protein and insulin signaling?

ACSS1 protein has been implicated in the regulation of insulin signaling and glucose metabolism in response to insulin.

What is the relationship between ACSS1 protein and the Warburg effect?

ACSS1 protein expression is upregulated in many cancer cells as part of the Warburg effect, which is a metabolic adaptation that allows cancer cells to produce energy through glycolysis.

What is the role of ACSS1 protein in autophagy?

ACSS1 protein has been implicated in the regulation of autophagy, a cellular process that degrades and recycles cellular components.

What is the role of ACSS1 protein in the brain?

ACSS1 protein is expressed in the brain and has been implicated in the regulation of neuronal function and synaptic plasticity.

What is the role of ACSS1 protein in adipocyte differentiation?

ACSS1 protein is required for the differentiation of adipocytes and the storage of triglycerides in adipose tissue.

What is the relationship between ACSS1 protein and glucose metabolism?

ACSS1 protein plays a critical role in linking glucose metabolism to fatty acid synthesis in the cytosol of mammalian cells.