Recombinant Full Length Human AEN Protein, GST-tagged

• Cat.No.: AEN-984HF
• Product Overview: Human AEN full-length ORF (AAH05164.1, 1 a.a. - 327 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: AEN (Apoptosis Enhancing Nuclease) is a Protein Coding gene. GO annotations related to this gene include nucleic acid binding and exonuclease activity. An important paralog of this gene is ISG20L2.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 62.37 kDa
• Protein Length: 327 amino acids
• AA Sequence: MVPREAPESA QCLCPSLTIP NAKDVLRKRH KRRSRQHQRF MARKALLQEQ GLLSMPPEPG SSPLPTPFGA ATATEAASSG KQCLRAGSGS APCSRRPAPG KASGPLPSKC VAIDCEMVGT GPRGRVSELA RCSIVSYHGD VLYDKYIRPE MPIADYRTRW SGITRQHMRK AVPFQVAQKE ILKLLKGKVV VGHALHNDFQ ALKYVHPRSQ TRDTTYVPNF LSEPGLHTRA RVSLKDLALQ LLHKKIQVGQ HGHSSVEDAT TAMELYRLVE VQWEQQEARS LWTCPEDREP DSSTDMEQYM EDQSTQYWAL KQKSEKQDSG LNSGAFV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AEN apoptosis enhancing nuclease [ Homo sapiens ]
• Official Symbol: AEN
• Synonyms: AEN; apoptosis enhancing nuclease; interferon stimulated exonuclease gene 20kDa like 1, ISG20L1; apoptosis-enhancing nuclease; FLJ12484; FLJ12562; interferon-stimulated 20 kDa exonuclease-like 1; interferon stimulated exonuclease gene 20kDa-like 1; ISG20L1; pp12744
• Gene ID: 64782
• mRNA Refseq: NM_022767
• Protein Refseq: NP_073604
• MIM: 610177
• UniProt ID: Q8WTP8

Reviews

08/30/2022

Exploring protein-protein interactions involved in neurotransmitter signaling.

05/24/2022

Characterizing protein-protein interactions involved in chromosomal organization.

08/20/2020

Determining protein-protein interaction interfaces using F枚rster resonance energy transfer.

Q&As

How is the subcellular localization of AEN protein determined, and are there specific mechanisms or targeting sequences involved?

The subcellular localization of AEN protein is determined by specific mechanisms and targeting sequences, which facilitate its transport and localization to specific cellular compartments.

How does dysregulation or dysfunction of AEN protein affect cellular functions or contribute to disease development, such as cancer or neurodegenerative disorders?

Dysregulation or dysfunction of AEN protein can disrupt cellular functions, such as impaired DNA repair or altered apoptotic responses, and contribute to the development of diseases like cancer or neurodegenerative disorders.

Are there any genetic variations or mutations in the AEN gene that impact the expression or function of AEN protein?

Genetic variations or mutations in the AEN gene may impact the expression or function of AEN protein, potentially influencing cellular processes or disease susceptibility.

Does AEN protein interact with other proteins or participate in protein complexes, and if so, what are the functional implications?

AEN protein may interact with other proteins, such as DNA repair factors or apoptotic regulators, forming protein complexes that influence cellular processes such as DNA repair or cell death.

What is the role of AEN protein in specific cellular processes or molecular pathways, such as DNA repair or apoptosis?

AEN protein is implicated in specific cellular processes such as DNA repair and apoptosis, where it may play a role in maintaining genomic stability or regulating cell survival.

What experimental techniques have been employed to investigate the functional significance of AEN protein?

Experimental techniques such as molecular biology, cell biology, and biochemical assays can be employed to investigate the functional significance of AEN protein, including DNA repair assays or apoptosis assays.

Are there any known post-translational modifications or regulatory factors that modulate the activity or stability of AEN protein?

Post-translational modifications or regulatory factors that modulate the activity or stability of AEN protein are not currently identified. Further research is necessary to explore these regulatory mechanisms.

Are there any specific structural features or domains of AEN protein that are critical for its function or interaction with other molecules?

The specific structural features or domains critical for the function or interaction of AEN protein are currently unknown, and further studies are needed to determine their role in cellular processes and molecular interactions.

What is the enzymatic activity or molecular function of AEN protein, and how is it measured or assessed in experimental assays?

The enzymatic activity or molecular function of AEN protein is currently unknown, and further experimental assays are needed to investigate its activity and molecular roles.

What are the tissue-specific expression patterns of AEN protein and the factors that regulate its expression levels?

The tissue-specific expression patterns of AEN protein vary across different organs and cell types. Factors regulating its expression levels include transcriptional control and signaling pathways.