Recombinant Full Length Human AMBRA1 Protein, C-Flag-tagged
Cat.No. : | AMBRA1-1736HFL |
Product Overview : | Recombinant Full Length Human AMBRA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. |
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Description : | Enables GTPase binding activity and ubiquitin protein ligase binding activity. Involved in macroautophagy; positive regulation of phosphatidylinositol 3-kinase activity; and response to mitochondrial depolarisation. Located in cytosol. Colocalizes with mitochondrion. Biomarker of multiple system atrophy. |
Source : | Mammalian cells |
Species : | Human |
Tag : | Flag |
Form : | 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Molecular Mass : | 132.7 kDa |
AA Sequence : | MKVVPEKNAVRILWGRERGARAMGA QRLLQELVEDKTRWMKWEGKRVELP DSPRSTFLLAFSPDRTLLASTHVNH NIYITEVKTGKCVHSLIGHRRTPWC VTFHPTISGLIASGCLDGEVRIWDL HGGSESWFTDSNNAIASLAFHPTAQ LLLIATANEIHFWDWSRREPFAVVK TASEMERVRLVRFDPLGHYLLTAIV NPSNQQGDDEPEIPIDGTELSHYRQ RALLQSQPVRRTPLLHNFLHMLSSR SSGIQVGEQSTVQDSATPSPPPPPP QPSTERPRTSAYIRLRQRVSYPTAE CCQHLGILCLCSRCSGTRVPSLLPH QDSVPPASARATTPSFSFVQTEPFH PPEQASSTQQDQGLLNRPSAFSTVQ SSTAGNTLRNLSLGPTRRSLGGPLS SHPSRYHREIAPGLTGSEWTRTVLS LNSRSEAESMPPPRTSASSVSLLSV LRQQEGGSQASVYTSATEGRGFPAS GLATESDGGNGSSQNNSGSIRHELQ CDLRRFFLEYDRLQELDQSLSGEAP QTQQAQEMLNNNIESERPGPSHQPT PHSSENNSNLSRGHLNRCRACHNLL TFNNDTLRWERTTPNYSSGEASSSW QVPSSFESVPSSGSQLPPLERTEGQ TPSSSRLELSSSASPQEERTVGVAF NQETGHWERIYTQSSRSGTVSQEAL HQDMPEESSEEDSLRRRLLESSLIS LSRYDGAGSREHPIYPDPARLSPAA YYAQRMIQYLSRRDSIRQRSMRYQQ NRLRSSTSSSSSDNQGPSVEGTDLE FEDFEDNGDRSRHRAPRNARMSAPS LGRFVPRRFLLPEYLPYAGIFHERG QPGLATHSSVNRVLAGAVIGDGQSA VASNIANTTYRLQWWDFTKFDLPEI SNASVNVLVQNCKIYNDASCDISAD GQLLAAFIPSSQRGFPDEGILAVYS LAPHNLGEMLYTKRFGPNAISVSLS PMGRYVMVGLASRRILLHPSTEHMV AQVFRLQQAHGGETSMRRVFNVLYP MPADQRRHVSINSARWLPEPGLGLA YGTNKGDLVICRPEALNSGVEYYWD QLNETVFTVHSNSRSSERPGTSRAT WRTDRDMGLMNAIGLQPRNPATSVT SQGTQTLALQLQNAETQTEREVPEP GTAASGPGEGEGSEYGASGEDALSR IQRLMAEGGMTAVVQREQSTTMASM GGFGNNIIVSHRIHRSSQTGTEPGA AHTSSPQPSTSRGLLPEAGQLAERG LSPRTASWDQPGTPGREPTQPTLPS SSPVPIPVSLPSAEGPTLHCELTNN NHLLDGGSSRGDAAGPRGEPRNRTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | >50 ug/mL as determined by microplate BCA method. |
Preparation : | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Gene Name : | AMBRA1 autophagy and beclin 1 regulator 1 [ Homo sapiens (human) ] |
Official Symbol : | AMBRA1 |
Synonyms : | DCAF3; WDR94 |
Gene ID : | 55626 |
mRNA Refseq : | NM_017749.3 |
Protein Refseq : | NP_060219.2 |
MIM : | 611359 |
UniProt ID : | Q9C0C7 |
Products Types
◆ Recombinant Protein | ||
AMBRA1-01H | Active Recombinant Human AMBRA1 Protein, Myc/DDK-tagged | +Inquiry |
AMBRA1-501M | Recombinant Mouse AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Ambra1-1608M | Recombinant Mouse Ambra1 Protein, Myc/DDK-tagged | +Inquiry |
AMBRA1-332H | Recombinant Human AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
AMBRA1-1590M | Recombinant Mouse AMBRA1 Protein | +Inquiry |
◆ Lysates | ||
AMBRA1-8887HCL | Recombinant Human AMBRA1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMBRA1 undergoes post-translational modifications, including phosphorylation and ubiquitination, which regulate its interaction with other autophagy-related proteins and modulate its function in autophagy.
AMBRA1 participates in selective autophagy processes, such as mitophagy (selective clearance of damaged mitochondria) and aggrephagy (clearance of protein aggregates), contributing to cellular quality control mechanisms.
AMBRA1 plays a critical role in autophagy regulation by promoting autophagosome formation, facilitating cargo recognition and degradation, and coordinating the activity of other autophagy-related proteins.
AMBRA1 facilitates the clearance of protein aggregates and damaged organelles through autophagy, preventing their accumulation and mitigating cellular damage.
AMBRA1 expression is induced during autophagy by various signaling pathways, including nutrient deprivation, cellular stress, and growth factor signaling.
In addition to its role in autophagy, AMBRA1 interacts with other cellular processes, such as apoptosis and cell proliferation, forming intricate crosstalk that influences cellular fate and homeostasis.
AMBRA1 interacts with Beclin 1, ULK1, and ATG proteins through protein-protein interactions, forming complexes that regulate autophagy initiation and autophagosome formation.
Depletion of AMBRA1 leads to impaired autophagy activity, resulting in the accumulation of damaged proteins and organelles and compromised cellular homeostasis.
AMBRA1 promotes autophagosome formation by regulating the nucleation and elongation steps, ensuring proper assembly of the autophagy machinery and cargo sequestration.
The activity of AMBRA1 in autophagy is modulated by upstream signaling pathways, including mTORC1, AMPK, and MAPK pathways, which integrate nutrient availability and cellular stresses.
Customer Reviews (3)
Write a reviewEasy integration into multi-omics workflows for comprehensive analysis.
Wide dynamic range for accurate quantification of protein changes.
Low false-positive rate in target protein identification.
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