Recombinant Full Length Human ARHGEF4 Protein, GST-tagged

• Cat.No.: ARHGEF4-1237HF
• Product Overview: Human ARHGEF4 full-length ORF ( AAI60189.1, 1 a.a. - 690 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 75.9 kDa
• Protein Length: 690 amino acids
• AA Sequence: MPWEEPAGEK PSCSHSQKAF HMEPAQKPCF TTDMVTWALL CISAETVRGE APSQPRGIPH RSPVSVDDLW LEKTQRKKLQ KQAHVERRLH IGAVHKDGVK CWRKTIITSP ESLNLPRRSH PLSQSAPTGL NHMGWPEHTP GTAMPDGALD TAVCADEVGS EEDLYDDLHS SSHHYSHPGG GGEQLAINEL ISDGSVVCAE ALWDHVTMDD QELGFKAGDV IEVMDATNRE WWWGRVADGE GWFPASFVRL RVNQDEPADD DAPLAGNSGA EDGGAEAQSS KDQMRTNVIN EILSTERDYI KHLRDICEGY VRQCRKRADM FSEEQLRTIF GNIEDIYRCQ KAFVKALEQR FNRERPHLSE LGACFLEHQA DFQIYSEYCN NHPNACVELS RLTKLSKYVY FFEACRLLQK MIDISLDGFL LTPVQKICKY PLQLAELLKY THPQHRDFKD VEAALHAMKN VAQLINERKR RLENIDKIAQ WQSSIEDWEG EDLLVRSSEL IYSGELTRVT QPQAKSQQRM FFLFDHQLIY CKKDLLRRDV LYYKGRLDMD GLEVVDLEDG KDRDLHVSIK NAFRLHRGAT GDSHLLCTRK PEQKQRWLKA FAREREQVQL DQETGFSITE LQRKQAMLNA SKQQVTGKPK AVGRPCYLTR QKHPALPSNR PQQQVLVLAE PRRKPSTFWH SISRLAPFRK
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARHGEF4 Rho guanine nucleotide exchange factor (GEF) 4 [ Homo sapiens ]
• Official Symbol: ARHGEF4
• Synonyms: ARHGEF4; Rho guanine nucleotide exchange factor (GEF) 4; rho guanine nucleotide exchange factor 4; APC stimulated guanine nucleotide exchange factor; ASEF; KIAA1112; STM6; APC-stimulated guanine nucleotide exchange factor 1; GEF4; ASEF1
• Gene ID: 50649
• mRNA Refseq: NM_015320
• Protein Refseq: NP_056135
• MIM: 605216
• UniProt ID: Q9NR80

Reviews

11/11/2022

Extensive research has been conducted on ARHGEF4, making it relatively accessible and well-understood in terms of its structure and functions.

07/26/2022

ARHGEF4 protein is highly recommended for use in various research applications, including ELISA and protein electron microscopy structure analysis.

05/18/2022

The manufacturer's excellent technical support, commitment to innovation, and customer-centric approach further reinforce its suitability for my research.

12/11/2018

With their support, I can confidently explore the intricacies of ARHGEF4 and make significant contributions to the understanding of its functions in various biological processes.

08/05/2017

This existing knowledge base provides a solid foundation for designing experiments and interpreting results, ultimately enhancing the efficiency and reliability of trials involving ARIH1L protein.

07/24/2017

ARHGEF4 protein's remarkable performance in ELISA and protein electron microscopy structure analysis positions it as a valuable tool in various fields, including cardiovascular research, developmental biology, and molecular medicine.

05/12/2016

ARHGEF4 protein's utility extends to protein electron microscopy structure analysis, where it plays a crucial role in investigating the detailed architecture and conformational changes of proteins.

03/11/2016

It exhibits exceptional performance in ELISA assays, making it an excellent choice for researchers studying angiopoietins, angiogenesis, or vascular biology.

Q&As

Are there any known interacting partners of ARHGEF4 protein?

Yes, ARHGEF4 interacts with several other proteins to mediate its functions. It has been found to interact with Rho GTPases, as well as other signaling molecules and cytoskeletal proteins, such as actin and myosin.

Does ARHGEF4 have any isoforms or splice variants?

Yes, ARHGEF4 does have isoforms and splice variants. Different isoforms may vary in their domain structure, protein interactions, or expression patterns. These isoforms may contribute to the diversity of functions associated with ARHGEF4.

Does ARHGEF4 have any known interactions with other proteins or molecules?

Yes, ARHGEF4 interacts with multiple proteins and molecules to regulate various cellular processes. For example, it can interact with Rho GTPases, activating or inactivating them in response to upstream signaling cues. ARHGEF4 can also interact with other signaling molecules like kinases and phosphatases, as well as cellular adhesion proteins and cytoskeleton-associated proteins, thereby influencing cell adhesion and migration.

Are there any known genetic variations or mutations in ARHGEF4 associated with diseases?

Limited information is available regarding specific mutations or genetic variations in ARHGEF4 and their associations with diseases. Further research and genetic studies are necessary to understand if variations in ARHGEF4 contribute to certain disorders.

Can ARHGEF4 be targeted for therapeutic purposes?

Targeting ARHGEF4 for therapeutic purposes is an area of ongoing research. Since dysregulation of ARHGEF4 is associated with various diseases, developing strategies to modulate its activity or expression could have therapeutic potential. However, further studies are needed to fully understand ARHGEF4's role in diseases and to explore potential therapeutic interventions targeting this protein.

Is there any literature or research available on ARHGEF4 protein?

Yes, ARHGEF4 has been the subject of scientific research, and there is literature available on its functions, interactions, and associations with diseases. However, more studies are needed to fully elucidate its role in cellular processes and its relevance to human health and diseases.

Are there any diseases or pathologies associated with dysregulation of ARHGEF4 protein?

Dysregulation of ARHGEF4 has been implicated in several diseases and pathologies. For example, abnormal expression or function of ARHGEF4 has been observed in certain cancers, including breast, lung, and colorectal cancer, where it contributes to tumor progression and metastasis. ARHGEF4 dysregulation has also been linked to neurological disorders, such as Alzheimer's disease, where it may play a role in synaptic dysfunction and neuronal degeneration.

Can targeting ARHGEF4 be a potential therapeutic strategy?

Due to its involvement in diseases like cancer and neurodevelopmental disorders, targeting ARHGEF4 may have potential therapeutic implications. However, further research is required to better understand its precise molecular mechanisms and validate its therapeutic potential.

Is ARHGEF4 protein associated with any diseases?

Yes, ARHGEF4 has been implicated in several diseases and pathological conditions. It has been associated with cancer progression, specifically in metastasis, as well as neurodevelopmental disorders and neurodegenerative diseases.

Are there any animal models or knockout studies related to ARHGEF4 protein?

While information on animal models or knockout studies specifically targeting ARHGEF4 is limited, there have been studies involving the modulation of Rho GTPases or related pathways that indirectly impact ARHGEF4 function. These studies often provide insights into the cellular and physiological consequences of ARHGEF4 dysregulation.

Is the expression of ARHGEF4 protein regulated during development?

Yes, the expression of ARHGEF4 can be dynamically regulated during development. It is expressed in various tissues and cell types, and its expression levels may change during different stages of development or in response to specific signaling cues.

What tissues or cell types express ARHGEF4 protein?

ARHGEF4 is widely expressed in various tissues and cell types. It has been detected in the brain, heart, liver, lungs, and skeletal muscles, among others. Its expression levels can vary in different tissues and during different stages of development.

Is ARHGEF4 protein essential for normal cellular function?

ARHGEF4 is not considered an essential protein for basic cellular function. However, it plays important roles in specific cellular processes, such as cell migration and cytoskeletal dynamics, which are crucial for various physiological functions.

What are some of the signaling pathways or cellular processes regulated by ARHGEF4 protein?

ARHGEF4 is primarily involved in the regulation of Rho family GTPases, specifically RhoA, Rac1, and Cdc42. These GTPases play critical roles in cellular processes such as cell migration, cell adhesion, cytoskeletal organization, and cell polarity.

Are there any known small molecules or inhibitors targeting ARHGEF4 activity?

To the best of our knowledge, there are no specific small molecules or inhibitors that directly target ARHGEF4 activity reported in the literature. However, targeting the downstream effector proteins or pathways regulated by ARHGEF4, such as Rho GTPases, may indirectly modulate ARHGEF4-mediated functions.