Recombinant Full Length Human ARRDC1 Protein, C-Flag-tagged

• Cat.No.: ARRDC1-1235HFL
• Product Overview: Recombinant Full Length Human ARRDC1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables several functions, including arrestin family protein binding activity; ubiquitin ligase-substrate adaptor activity; and ubiquitin protein ligase binding activity. Involved in several processes, including cellular protein metabolic process; extracellular vesicle biogenesis; and negative regulation of Notch signaling pathway. Located in cytoplasmic vesicle; extracellular vesicle; and plasma membrane.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.8 kDa
• AA Sequence: MGRVQLFEISLSHGRVVYSPGEPLA GTVRVRLGAPLPFRAIRVTCIGSCG VSNKANDTAWVVEEGYFNSS LSLADKGSLPAGEHSFPFQFLLPAT APTSFEGPFGKIVHQVRAAIHTPRF SKDHKCSLVFYILSPLNLNS IPDIEQPNVASATKKFSYKLVKTGS VVLTASTDLRGYVVGQALQLHADVE NQSGKDTSPVVASLLQKVSY KAKRWIHDVRTIAEVEGAGVKAWRR AQWHEQILVPALPQSALPGCSLIHI DYYLQVSLKAPEATVTLPVF IGNIAVNHAPVSPRPGLGLPPGAPP LVVPSAPPQEEAEAEAAAGGPHFLD PVFLSTKSHSQRQPLLATLS SVPGAPEPCPQDGSPASHPLHPPLC ISTGATVPYFAEGSGGPVPTTSTLI LPPEYSSWGYPYEAPPSYEQ
  SCGGVEPSLTPESTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: ARRDC1 arrestin domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: ARRDC1
• Synonyms: RP11-48C7.5
• Gene ID: 92714
• mRNA Refseq: NM_152285.4
• Protein Refseq: NP_689498.1
• MIM: 619768
• UniProt ID: Q8N5I2

Reviews

06/17/2022

I am confident that utilizing the ARRDC1 protein will empower me to uncover crucial insights into cellular mechanisms with precision.

01/16/2022

The ARRDC1 protein is an exceptional protein solution that exceeds expectations in terms of quality and suitability for my experimental requirements.

06/09/2016

Their expertise and guidance will undoubtedly prove valuable in overcoming hurdles and maximizing the potential of this protein in my research endeavors.

Q&As

Can targeting ARRDC1 be a potential therapeutic strategy for cardiovascular diseases?

Modulating the activity of ARRDC1 or its interaction with receptors could be a potential therapeutic strategy for cardiovascular diseases. By targeting ARRDC1, it may be possible to attenuate cardiac hypertrophy and prevent the progression of heart failure. However, further research is needed to fully understand the underlying mechanisms and determine the feasibility and efficacy of targeting ARRDC1 in the treatment of cardiovascular diseases.

Can ARRDC1 be targeted therapeutically?

Currently, there are no specific drugs or therapeutic agents that directly target ARRDC1. However, understanding the functional significance of ARRDC1 in disease processes may guide the development of novel therapeutic strategies in the future. Modulating ARRDC1 activity or its interaction with specific receptors could have therapeutic benefits in diseases where ARRDC1 dysregulation plays a role.

How does ARRDC1 regulate receptor signaling?

ARRDC1 can regulate receptor signaling through multiple mechanisms. It can directly bind to receptors and promote their internalization, leading to receptor desensitization. ARRDC1 can also recruit other proteins involved in receptor signaling pathways, thereby influencing downstream signaling events. Additionally, ARRDC1 has been shown to regulate the levels and stability of certain receptors by promoting their ubiquitination and targeting them for degradation.

Which receptors does ARRDC1 interact with?

ARRDC1 has been found to interact with a variety of receptors, including beta-adrenergic receptors, angiotensin receptors, platelet-derived growth factor receptors, and epidermal growth factor receptors. These interactions are important for the regulation of receptor signaling and downstream cellular responses.

Are there any diseases or conditions associated with ARRDC1 dysfunction?

There is emerging evidence to suggest that ARRDC1 dysregulation may be associated with various diseases and conditions. For example, altered expression levels of ARRDC1 have been observed in certain types of cancer, including lung and breast cancer. Furthermore, aberrant ARRDC1 signaling has been implicated in cardiac hypertrophy and heart failure.

Is ARRDC1 conserved across different species?

Yes, ARRDC1 is evolutionarily conserved across different species, including humans, mice, rats, and other organisms. This conservation suggests its importance in fundamental cellular processes and indicates potential functional significance across different organisms.

How is the expression of ARRDC1 regulated?

The expression of ARRDC1 can be regulated at both transcriptional and post-transcriptional levels. It has been reported that certain signaling pathways, such as the β-adrenergic signaling pathway, can upregulate ARRDC1 expression. Additionally, microRNAs have been identified that can bind to the ARRDC1 mRNA and regulate its stability, leading to changes in protein expression.