Recombinant Full Length Human DNAJA2 Protein, C-Flag-tagged

• Cat.No.: DNAJA2-1657HFL
• Product Overview: Recombinant Full Length Human DNAJA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.6 kDa
• AA Sequence: MANVADTKLYDILGVPPGASENELK KAYRKLAKEYHPDKNPNAGDKFKEI SFAYEVLSNPEKRELYDRYG EQGLREGSGGGGGMDDIFSHIFGGG LFGFMGNQSRSRNGRRRGEDMMHPL KVSLEDLYNGKTTKLQLSKN VLCSACSGQGGKSGAVQKCSACRGR GVRIMIRQLAPGMVQQMQSVCSDCN GEGEVINEKDRCKKCEGKKV IKEVKILEVHVDKGMKHGQRITFTG EADQAPGVEPGDIVLLLQEKEHEVF QRDGNDLHMTYKIGLVEALC GFQFTFKHLDGRQIVVKYPPGKVIE PGCVRVVRGEGMPQYRNPFEKGDLY IKFDVQFPENNWINPDKLSE
  LEDLLPSRPEVPNIIGETEEVELQE FDSTRGSGGGQRREAYNDSSDEESS SHHGPGVQCAHQTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DNAJA2 DnaJ heat shock protein family (Hsp40) member A2 [ Homo sapiens (human) ]
• Official Symbol: DNAJA2
• Synonyms: DJ3; CPR3; DJA2; DNAJ; DNJ3; RDJ2; HIRIP4; PRO3015
• Gene ID: 10294
• mRNA Refseq: NM_005880.4
• Protein Refseq: NP_005871.1
• MIM: 611322
• UniProt ID: O60884

Reviews

11/14/2022

Cheap price, high quality products, worth buying again.

04/01/2022

The product was kept in perfect condition and delivered on time so that I got the perfect product in the shortest time possible.

11/26/2021

I have a dedicated staff member who is in contact with me and he keep me informed of the progress of my experiments.

Q&As

What are the biological functions of DNAJA2?

DNAJA2 can act as a synergistic molecular chaperone to help cellular protein translation, folding, degradation, etc.

What are the effects of overexpressing DNAJA2?

Overexpression of DNAJA2 promotes JEV proliferation.

What is the relationship between DNAJA1 and DNAJA2?

They both belong to the DnaJ family of A-type proteins and they can interact with each other.

How is DNAJA2 related to NS3?

DNAJA2 interacts with NS3.

What effect does inhibiting the expression of DNAJA2 have on NS3 and NS5?

Inhibition of DNAJA2 expression promotes ubiquitination modification of NS3 and NS5.