Recombinant Human DNAJA2 293 Cell Lysate

• Cat.No.: DNAJA2-6894HCL

Specification

• Description: Antigen standard for DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DNAJA2 DnaJ (Hsp40) homolog, subfamily A, member 2 [ Homo sapiens ]
• Official Symbol: DNAJA2
• Synonyms: DNAJA2; DnaJ (Hsp40) homolog, subfamily A, member 2; dnaJ homolog subfamily A member 2; CPR3; DNAJ; DNJ3; HIRIP4; HIRA interacting protein 4; HIRA-interacting protein 4; cell cycle progression 3 protein; renal carcinoma antigen NY-REN-14; cell cycle progression restoration gene 3 protein; DJ3; DJA2; RDJ2; PRO3015
• Gene ID: 10294
• mRNA Refseq: NM_005880
• Protein Refseq: NP_005871
• MIM: 611322
• UniProt ID: O60884
• Chromosome Location: 16q12.1
• Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem
• Function: ATP binding; glycoprotein binding; heat shock protein binding; metal ion binding; unfolded protein binding

Reviews

11/14/2022

Cheap price, high quality products, worth buying again.

04/01/2022

The product was kept in perfect condition and delivered on time so that I got the perfect product in the shortest time possible.

11/26/2021

I have a dedicated staff member who is in contact with me and he keep me informed of the progress of my experiments.

Q&As

What are the biological functions of DNAJA2?

DNAJA2 can act as a synergistic molecular chaperone to help cellular protein translation, folding, degradation, etc.

What are the effects of overexpressing DNAJA2?

Overexpression of DNAJA2 promotes JEV proliferation.

What is the relationship between DNAJA1 and DNAJA2?

They both belong to the DnaJ family of A-type proteins and they can interact with each other.

How is DNAJA2 related to NS3?

DNAJA2 interacts with NS3.

What effect does inhibiting the expression of DNAJA2 have on NS3 and NS5?

Inhibition of DNAJA2 expression promotes ubiquitination modification of NS3 and NS5.