Recombinant Full Length Human GUSB Protein, C-Flag-tagged

• Cat.No.: GUSB-2155HFL
• Product Overview: Recombinant Full Length Human GUSB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 72.5 kDa
• AA Sequence: MARGSAVAWAALGPLLWGCALGLQG GMLYPQESPSRECKELDGLWSFRAD FSDNRRRGFEEQWYRRPLWE SGPTVDMPVPSSFNDISQDWRLRHF VGWVWYEREVILPERWTQDLRTRVV LRIGSAHSYAIVWVNGVDTL EHEGGYLPFEADISNLVQVGPLPSR LRITIAINNTLTPTTLPPGTIQYLT DTSKYPKGYFVQNTYFDFFN YAGLQRSVLLYTTPTTYIDDITVTT SVEQDSGLVNYQISVKGSNLFKLEV RLLDAENKVVANGTGTQGQL KVPGVSLWWPYLMHERPAYLYSLEV QLTAQTSLGPVSDFYTLPVGIRTVA VTKSQFLINGKPFYFHGVNK HEDADIRGKGFDWPLLVKDFNLLRW LGANAFRTSHYPYAEEVMQMCDRYG IVVIDECPGVGLALPQFFNN VSLHHHMQVMEEVVRRDKNHPAVVM WSVANEPASHLESAGYYLKMVIAHT KSLDPSRPVTFVSNSNYAAD KGAPYVDVICLNSYYSWYHDYGHLE LIQLQLATQFENWYKKYQKPIIQSE YGAETIAGFHQDPPLMFTEE YQKSLLEQYHLGLDQKRRKYVVGEL IWNFADFMTEQSPTRVLGNKKGIFT RQRQPKSAAFLLRERYWKIA NETRYPHSVAKSQCLENSLFT TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - other enzymes, Glycosaminoglycan degradation, Lysosome, Metabolic pathways, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Starch and sucrose metabolism

Gene Information

• Gene Name: GUSB glucuronidase beta [ Homo sapiens (human) ]
• Official Symbol: GUSB
• Synonyms: BG; MPS7
• Gene ID: 2990
• mRNA Refseq: NM_000181.4
• Protein Refseq: NP_000172.2
• MIM: 611499
• UniProt ID: P08236