Recombinant Full Length Human Long-Chain-Fatty-Acid--Coa Ligase 3(Acsl3) Protein, His-Tagged

• Cat.No.: RFL-34899HF
• Product Overview: Recombinant Full Length Human Long-chain-fatty-acid--CoA ligase 3(ACSL3) Protein (O95573) (1-720aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Homo sapiens (Human)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-720)
• AA Sequence: MNNHVSSKPSTMKLKHTINPILLYF IHFLISLYTILTYIPFYFFSESRQE KSNRIKAKPVNSKPDSAYRSVNSLD GLASVLYPGCDTLDKVFTYAKNKFK NKRLLGTREVLNEEDEVQPNGKIFK KVILGQYNWLSYEDVFVRAFNFGNG LQMLGQKPKTNIAIFCETRAEWMIA AQACFMYNFQLVTLYATLGGPAIVH ALNETEVTNIITSKELLQTKLKDIV SLVPRLRHIITVDGKPPTWSEFPKG IIVHTMAAVEALGAKASMENQPHSK PLPSDIAVIMYTSGSTGLPKGVMIS HSNIIAGITGMAERIPELGEEDVYI GYLPLAHVLELSAELVCLSHGCRIG YSSPQTLADQSSKIKKGSKGDTSML KPTLMAAVPEIMDRIYKNVMNKVSE MSSFQRNLFILAYNYKMEQISKGRN TPLCDSFVFRKVRSLLGGNIRLLLC GGAPLSATTQRFMNICFCCPVGQGY GLTESAGAGTISEVWDYNTGRVGAP LVCCEIKLKNWEEGGYFNTDKPHPR GEILIGGQSVTMGYYKNEAKTKADF FEDENGQRWLCTGDIGEFEPDGCLK IIDRKKDLVKLQAGEYVSLGKVEAA LKNLPLVDNICAYANSYHSYVIGFV VPNQKELTELARKKGLKGTWEELCN SCEMENEVLKVLSEAAISASLEKFE IPVKIRLSPEPWTPETGLVTDAFKL KRKELKTHYQADIERMYGRK
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: ACSL3
• Synonyms: ACSL3; ACS3; FACL3; LACS3; Fatty acid CoA ligase Acsl3; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 3; LACS 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3
• UniProt ID: O95573

Gene Information

• Gene Name: ACSL3
• Synonyms: ACSL3; ACS3; FACL3; LACS3; Fatty acid CoA ligase Acsl3; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 3; LACS 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3
• UniProt ID: O95573

Reviews

06/14/2021

The product was well-characterized, and the documentation provided was thorough and helpful.

08/27/2020

The protein arrived quickly and was exactly what I needed for my experiment. Very satisfied with my purchase.

06/01/2020

The website was easy to navigate and the ordering process was simple. Appreciate the user-friendly interface.

10/04/2019

I'm really pleased with my purchase of the recombinant protein. It's high quality and reasonably priced.

09/01/2018

I appreciate the transparency and honesty of this company in their communication with customers. Will continue to order from them in the future.

Q&As

What is the relationship between ACSL3 and cardiovascular disease?

ACSL3 dysregulation has been linked to the development of cardiovascular disease, highlighting the importance of the gene in the regulation of lipid metabolism and cardiovascular health.

What is the impact of ACSL3 on autophagy?

ACSL3 has been shown to impact autophagy, a cellular process involved in the breakdown and recycling of damaged or dysfunctional cellular components.

What is the relationship between ACSL3 and insulin resistance?

ACSL3 dysregulation has been implicated in the development of insulin resistance, a common feature of many metabolic disorders.

What is the impact of ACSL3 on adipogenesis?

ACSL3 has been shown to impact adipogenesis, the process of fat cell differentiation, by regulating the availability of long-chain fatty acids for lipid synthesis and storage.

What is the structure of the ACSL3 protein?

The ACSL3 protein consists of multiple domains, including an N-terminal domain, a central domain, and a C-terminal domain, which are involved in substrate binding, acyl-adenylate formation, and ATP binding, respectively.

What is the impact of ACSL3 on cellular signaling pathways?

ACSL3 has been shown to impact cellular signaling pathways, particularly those involved in the regulation of inflammation, insulin signaling, and energy metabolism.

What is the role of ACSL3 in lipid metabolism?

ACSL3 plays a critical role in lipid metabolism by activating long-chain fatty acids for subsequent utilization in various metabolic pathways, including β-oxidation and the production of signaling lipids.

What is the expression pattern of ACSL3 in different tissues?

ACSL3 is expressed in a variety of tissues, including adipose tissue, liver, muscle, heart, and brain, with highest expression levels observed in adipose tissue.

How does ACSL3 impact lipid droplet formation?

ACSL3 has been shown to impact lipid droplet formation, with increased expression of the gene leading to enhanced lipid droplet formation and storage.

How does ACSL3 impact cellular proliferation and differentiation?

ACSL3 has been shown to impact cellular proliferation and differentiation by modulating the availability of long-chain fatty acids for various cellular processes.