Recombinant Full Length Human MYH6 Protein, C-Flag-tagged

• Cat.No.: MYH6-451HFL
• Product Overview: Recombinant Full Length Human MYH6 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 223.6 kDa
• AA Sequence: MTDAQMADFGAAAQYLRKSEKERLE AQTRPFDIRTECFVPDDKEEFVKAK ILSREGGKVIAETENGKTVT VKEDQVLQQNPPKFDKIEDMAMLTF LHEPAVLFNLKERYAAWMIYTYSGL FCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDNAYQYM LTDRENQSILITGESGAGKTVNTKR VIQYFASIAAIGDRGKKDNA NANKGTLEDQIIQANPALEAFGNAK TVRNDNSSRFGKFIRIHFGATGKLA SADIETYLLEKSRVIFQLKA ERNYHIFYQILSNKKPELLDMLLVT NNPYDYAFVSQGEVSVASIDDSEEL MATDSAFDVLGFTSEEKAGV YKLTGAIMHYGNMKFKQKQREEQAE PDGTEDADKSAYLMGLNSADLLKGL CHPRVKVGNEYVTKGQSVQQ VYYSIGALAKAVYEKMFNWMVTRIN ATLETKQPRQYFIGVLDIAGFEIFD FNSFEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWTFIDFG MDLQACIDLIEKPMGIMSILEEECM FPKATDMTFKAKLYDNHLGK SNNFQKPRNIKGKQEAHFSLIHYAG TVDYNILGWLEKNKDPLNETVVALY QKSSLKLMATLFSSYATADT GDSGKSKGGKKKGSSFQTVSALHRE NLNKLMTNLRTTHPHFVRCIIPNER KAPGVMDNPLVMHQLRCNGV LEGIRICRKGFPNRILYGDFRQRYR ILNPVAIPEGQFIDSRKGTEKLLSS LDIDHNQYKFGHTKVFFKAG LLGLLEEMRDERLSRIITRMQAQAR GQLMRIEFKKIVERRDALLVIQWNI RAFMGVKNWPWMKLYFKIKP LLKSAETEKEMATMKEEFGRIKETL EKSEARRKELEEKMVSLLQEKNDLQ LQVQAEQDNLNDAEERCDQL IKNKIQLEAKVKEMNERLEDEEEMN AELTAKKRKLEDECSELKKDIDDLE LTLAKVEKEKHATENKVKNL TEEMAGLDEIIAKLTKEKKALQEAH QQALDDLQVEEDKVNSLSKSKVKLE QQVDDLEGSLEQEKKVRMDL ERAKRKLEGDLKLTQESIMDLENDK LQLEEKLKKKEFDINQQNSKIEDEQ VLALQLQKKLKENQARIEEL EEELEAERTARAKVEKLRSDLSREL EEISERLEEAGGATSVQIEMNKKRE AEFQKMRRDLEEATLQHEAT AAALRKKHADSVAELGEQIDNLQRV KQKLEKEKSEFKLELDDVTSNMEQI IKAKANLEKVSRTLEDQANE YRVKLEEAQRSLNDFTTQRAKLQTE NGELARQLEEKEALISQLTRGKLSY TQQMEDLKRQLEEEGKAKNA LAHALQSARHDCDLLREQYEEETEA KAELQRVLSKANSEVAQWRTKYETD AIQRTEELEEAKKKLAQRLQ DAEEAVEAVNAKCSSLEKTKHRLQN EIEDLMVDVERSNAAAAALDKKQRN FDKILAEWKQKYEESQSELE SSQKEARSLSTELFKLKNAYEESLE HLETFKRENKNLQEEISDLTEQLGE GGKNVHELEKVRKQLEVEKL ELQSALEEAEASLEHEEGKILRAQL EFNQIKAEIERKLAEKDEEMEQAKR NHQRVVDSLQTSLDAETRSR NEVLRVKKKMEGDLNEMEIQLSHAN RMAAEAQKQVKSLQSLLKDTQIQLD DAVRANDDLKENIAIVERRN NLLQAELEELRAVVEQTERSRKLAE QELIETSERVQLLHSQNTSLINQKK KMESDLTQLQSEVEEAVQEC RNAEEKAKKAITDAAMMAEELKKEQ DTSAHLERMKKNMEQTIKDLQHRLD EAEQIALKGGKKQLQKLEAR VRELEGELEAEQKRNAESVKGMRKS ERRIKELTYQTEEDKKNLLRLQDLV DKLQLKVKAYKRQAEEAEEQ
  ANTNLSKFRKVQHELDEAEERADIA ESQVNKLRAKSRDIGAKQKMHDEET RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Tight junction, Viral myocarditis

Gene Information

• Gene Name: MYH6 myosin heavy chain 6 [ Homo sapiens (human) ]
• Official Symbol: MYH6
• Synonyms: ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• Gene ID: 4624
• mRNA Refseq: NM_002471.4
• Protein Refseq: NP_002462.2
• MIM: 160710
• UniProt ID: P13533