Recombinant Full Length Human NUMB Protein, C-Flag-tagged

• Cat.No.: NUMB-911HFL
• Product Overview: Recombinant Full Length Human NUMB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.6 kDa
• AA Sequence: MNKLRQSFRRKKDVYVPEASRPHQW QTDEEGVRTGKCSFPVKYLGHVEVD ESRGMHICEDAVKRLKAERK FFKGFFGKTGKKAVKAVLWVSADGL RVVDEKTKDLIVDQTIEKVSFCAPD RNFDRAFSYICRDGTTRRWI CHCFMAVKDTGERLSHAVGCAFAAC LERKQKREKECGVTATFDASRTTFT REGSFRVTTATEQAKREEIM KQMQDAKKAETDKIVVGSSVAPGNT APSPSSPTSPTSDATTSLEMNNPHA IPRRHAPIEQLARQGSFRGF PALSQKMSPFKRQLSLRINELPSTM QRKTDFPIKNAVPEVEGEAESISSL CSQITNAFSTPEDPFSSAPM TKPVTVVAPQSPTFQANGTDSAFHV LAKPAHTALAPVAMPVRETNPWAHA PDAANKEIAATCSGTEWGQS SGAASPGLFQAGHRRTPSEADRWLE EVSKSVRAQQPQASAAPLQPVLQPP PPTAISQPASPFQGNAFLTS QPVPVGVVPALQPAFVPAQSYPVAN GMPYPAPNVPVVGITPSQMVANVFG TAGHPQAAHPHQSPSLVRQQ TFPHYEASSATTSPFFKPPAQHLNG SAAFNGVDDGRLASADRHTEVPTGT CPVDPFEAQWAALENKSKQR
  TNPSPTNPFSSDLQKTFEIELTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Notch signaling pathway

Gene Information

• Gene Name: NUMB NUMB endocytic adaptor protein [ Homo sapiens (human) ]
• Official Symbol: NUMB
• Synonyms: S171; C14orf41; c14_5527
• Gene ID: 8650
• mRNA Refseq: NM_001005743.2
• Protein Refseq: NP_001005743.1
• MIM: 603728
• UniProt ID: P49757